Hyperchromic effect
The hyperchromic effect occurs when the absorption area of the DNA is increased by heating and light in the range from 200 to 280 nm is increasingly absorbed as a result.
When the DNA is heated, the double helix increasingly separates . This reduces the interactions of the π electrons and increases the absorption area of the incident light. This effect is relevant in the context of Fluometrie (see also fluorescence ), since by the effect of the change fluometrischen properties and so the guanine - cytosine content of DNA can be determined.
See also
swell
- Thermodynamic investigation ( memento from June 29, 2007 in the Internet Archive ) script, Technical University of Darmstadt
- Winter, Roland; Noll, Frank : Methods of Biophysical Chemistry, Teubner Study Books Chemistry (1st edition, Stuttgart 1998)