Artificial DNA

Artificial DNA (also known as synthetic DNA ) refers to DNA that does not occur naturally. It can be generated by various methods of DNA synthesis, such as phosphoramidite synthesis (for oligonucleotides ), the polymerase chain reaction (for polynucleotides with an existing template) or artificial gene synthesis (for polynucleotides without an existing template). Synthetic DNA is used for research or DNA property marking purposes. In addition to nature-identical synthetic DNA, artificial DNA also includes synthetic DNA analogues such as LNA and PNA . Synthetic deoxyribozymes are also included in artificial DNA.

literature

Randall A. Hughes: Synthetic DNA. Springer New York, 2018, ISBN 978-1-493-98170-0 .
Dan Luo: Synthetic DNA Delivery Systems. Springer Science & Business Media, 2003, ISBN 978-0-306-47701-0 , p. 13.
Yury E. Khudyakov: Artificial DNA. CRC Press, 2002, ISBN 978-1-420-04016-6 .

Individual evidence

^ RA Hughes, AD Ellington: Synthetic DNA Synthesis and Assembly: Putting the Synthetic in Synthetic Biology. In: Cold Spring Harbor perspectives in biology. Volume 9, number 1, January 2017, p., Doi : 10.1101 / cshperspect.a023812 , PMID 28049645 , PMC 5204324 (free full text).
↑ A. Grajkowski, J. Cieślak, SL Beaucage: A high-throughput process for the solid-phase Purification of Synthetic DNA Sequences. In: Current protocols in nucleic acid chemistry. Volume 69, 06 2017, pp. 10.17.1-10.17.30, doi : 10.1002 / cpnc.31 , PMID 28628204 , PMC 5568675 (free full text).
↑ DS Lee, H. Qian, CY Tay, DT Leong: Cellular processing and destinies of artificial DNA nanostructures. In: Chemical Society reviews. Volume 45, Number 15, August 2016, pp. 4199-4225, doi : 10.1039 / c5cs00700c , PMID 27119124 .
↑ YH Lau, F. Stirling, J. Kuo, MA Karrenbelt, YA Chan, A. Riesselman, CA Horton, E. Schäfer, D. Lips, MT Weinstock, DG Gibson, JC Way, PA Silver: Large-scale recoding of a bacterial genome by iterative recombineering of synthetic DNA. In: Nucleic acids research. Volume 45, number 11, June 2017, pp. 6971-6980, doi : 10.1093 / nar / gkx415 , PMID 28499033 , PMC 5499800 (free full text).
↑ Andreas Ulrich: British miracle tincture . In: Der Spiegel . No. 11 , 2009, p. 60 ( online - October 18, 2009 ).
^ R. D'Agata, G. Spoto: Artificial DNA and surface plasmon resonance. In: Artificial DNA, PNA & XNA. Volume 3, number 2, 2012 Apr-Jun, pp. 45-52, doi : 10.4161 / adna.21383 , PMID 22821257 , PMC 3429530 (free full text).
^ SK Silverman: Catalytic DNA: Scope, Applications, and Biochemistry of Deoxyribozymes. In: Trends in Biochemical Sciences . Volume 41, number 7, 07 2016, pp. 595-609, doi : 10.1016 / j.tibs.2016.04.010 , PMID 27236301 , PMC 4930396 (free full text).

[1] RA Hughes, AD Ellington: Synthetic DNA Synthesis and Assembly: Putting the Synthetic in Synthetic Biology. In: Cold Spring Harbor perspectives in biology. Volume 9, number 1, January 2017, p., Doi : 10.1101 / cshperspect.a023812 , PMID 28049645 , PMC 5204324 (free full text).

[2] A. Grajkowski, J. Cieślak, SL Beaucage: A high-throughput process for the solid-phase Purification of Synthetic DNA Sequences. In: Current protocols in nucleic acid chemistry. Volume 69, 06 2017, pp. 10.17.1-10.17.30, doi : 10.1002 / cpnc.31 , PMID 28628204 , PMC 5568675 (free full text).

[3] DS Lee, H. Qian, CY Tay, DT Leong: Cellular processing and destinies of artificial DNA nanostructures. In: Chemical Society reviews. Volume 45, Number 15, August 2016, pp. 4199-4225, doi : 10.1039 / c5cs00700c , PMID 27119124 .

[4] YH Lau, F. Stirling, J. Kuo, MA Karrenbelt, YA Chan, A. Riesselman, CA Horton, E. Schäfer, D. Lips, MT Weinstock, DG Gibson, JC Way, PA Silver: Large-scale recoding of a bacterial genome by iterative recombineering of synthetic DNA. In: Nucleic acids research. Volume 45, number 11, June 2017, pp. 6971-6980, doi : 10.1093 / nar / gkx415 , PMID 28499033 , PMC 5499800 (free full text).

[5] Andreas Ulrich: British miracle tincture . In: Der Spiegel . No. 11 , 2009, p. 60 ( online - October 18, 2009 ).

[6] R. D'Agata, G. Spoto: Artificial DNA and surface plasmon resonance. In: Artificial DNA, PNA & XNA. Volume 3, number 2, 2012 Apr-Jun, pp. 45-52, doi : 10.4161 / adna.21383 , PMID 22821257 , PMC 3429530 (free full text).

[7] SK Silverman: Catalytic DNA: Scope, Applications, and Biochemistry of Deoxyribozymes. In: Trends in Biochemical Sciences . Volume 41, number 7, 07 2016, pp. 595-609, doi : 10.1016 / j.tibs.2016.04.010 , PMID 27236301 , PMC 4930396 (free full text).