lin-28

lin-28 (also lin28) is a developmental gene found in the cells of most multicellular animals. Its position in the human genome is on chromosome 1, location 1p36.11. The protein product of lin-28 is an evolutionarily highly conserved, RNA-binding protein that is regulated by microRNAs and is important in the timing of the embryonic development of Caenorhabditis elegans . The name for the gene comes from English and means "cell lineage abnormally 28" ( "abnormal cell lineage 28").

The protein product is a so-called transcription factor (TF), which causes the reading of other genes. The TF contains a zinc finger and a cold shock domain (csd). After completion of the development of the organism, lin-28 is completely switched off .

The gene serves as a marker for undifferentiated human embryonic stem cells and is used in biotechnology to produce artificial stem cells ( induced pluripotent stem cells ). It codes for a cytoplasmic mRNA binding protein that helps translate the mRNA from another gene, Igf2 .

It has also been shown that lin-28 binds to precursors of a non-coding RNA that is involved in the control of developmental processes ( Let-7 ) and thus blocks the production of the mature let-7 molecule in mouse embryonic stem cells.

Individual evidence

↑ Horvitz HR, Sternberg PW, Greenwald IS, Fixsen W, Ellis HM: Mutations that affect neural cell lineages and cell fates during the development of the nematode Caenorhabditis elegans . In: Quant Biol. . 48, No. 2, 1983, pp. 453-463. PMID 6586368 .
^ Moss EG, Tang L. Conservation of the heterochronic regulator Lin-28, its developmental expression and microRNA complementary sites. In: Dev Biol . 2003; 258: 432-442
^ Moss EG, Lee RC, Ambros V .: The cold shock domain protein LIN-28 controls developmental timing in C. elegans and is regulated by the lin-4 RNA . In: Cell . 88, No. 5, 1997 Mar 7, pp. 637-646. PMID 9054503 .
↑ Richards M, Tan SP, Tan JH, Chan WK, Bongso A .: The transcriptome profile of human embryonic stem cells as defined by SAGE . In: Stem Cells . 22, No. 1, 2004, pp. 51-64. PMID 14688391 .
↑ Liao J, Wu Z, Wang Y, Cheng L, Cui C, Gao Y, Chen T, Rao L, Chen S, Jia N, Dai H, Xin S, Kang J, Pei G, Xiao L: Enhanced efficiency of generating induced pluripotent stem (iPS) cells from human somatic cells by a combination of six transcription factors . In: Cell Res . 18, No. 5, 2008 May, pp. 600-603. PMID 18414447 .
↑ Balzer E, Moss EG: Localization of the developmental timing regulator Lin28 to mRNP complexes, P-bodies and stress granules . In: RNA Biol . 4, No. 1, 2007 Jan-Mar, pp. 16-25. PMID 17617744 .
↑ Polesskaya A, Cuvellier S, Naguibneva I, Duquet A, Moss EG, Harel-Bellan A .: Lin-28 binds IGF-2 mRNA and participates in skeletal myogenesis by increasing translation efficiency . In: Genes Dev . 1, No. 9, 2007 May 1, pp. 1125-1138. PMID 17473174 .
↑ Viswanathan SR, Daley GQ, Gregory RI: Selective blockade of microRNA processing by Lin28 . In: Science . 320, No. 5872, 2008 Apr 4, pp. 97-100. PMID 18292307 .

[Horvitz-1] Horvitz HR, Sternberg PW, Greenwald IS, Fixsen W, Ellis HM: Mutations that affect neural cell lineages and cell fates during the development of the nematode Caenorhabditis elegans . In: Quant Biol. . 48, No. 2, 1983, pp. 453-463. PMID 6586368 .

[2] Moss EG, Tang L. Conservation of the heterochronic regulator Lin-28, its developmental expression and microRNA complementary sites. In: Dev Biol . 2003; 258: 432-442

[3] Moss EG, Lee RC, Ambros V .: The cold shock domain protein LIN-28 controls developmental timing in C. elegans and is regulated by the lin-4 RNA . In: Cell . 88, No. 5, 1997 Mar 7, pp. 637-646. PMID 9054503 .

[4] Richards M, Tan SP, Tan JH, Chan WK, Bongso A .: The transcriptome profile of human embryonic stem cells as defined by SAGE . In: Stem Cells . 22, No. 1, 2004, pp. 51-64. PMID 14688391 .

[5] Liao J, Wu Z, Wang Y, Cheng L, Cui C, Gao Y, Chen T, Rao L, Chen S, Jia N, Dai H, Xin S, Kang J, Pei G, Xiao L: Enhanced efficiency of generating induced pluripotent stem (iPS) cells from human somatic cells by a combination of six transcription factors . In: Cell Res . 18, No. 5, 2008 May, pp. 600-603. PMID 18414447 .

[6] Balzer E, Moss EG: Localization of the developmental timing regulator Lin28 to mRNP complexes, P-bodies and stress granules . In: RNA Biol . 4, No. 1, 2007 Jan-Mar, pp. 16-25. PMID 17617744 .

[7] Polesskaya A, Cuvellier S, Naguibneva I, Duquet A, Moss EG, Harel-Bellan A .: Lin-28 binds IGF-2 mRNA and participates in skeletal myogenesis by increasing translation efficiency . In: Genes Dev . 1, No. 9, 2007 May 1, pp. 1125-1138. PMID 17473174 .

[8] Viswanathan SR, Daley GQ, Gregory RI: Selective blockade of microRNA processing by Lin28 . In: Science . 320, No. 5872, 2008 Apr 4, pp. 97-100. PMID 18292307 .