Microantibodies

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A microantibody is a peptide derived from an antibody or from a phage display that binds to the same antigen .

properties

Microantibodies are peptides derived from the antigen-binding amino acid sequence of an antibody or derived from a phage display. They consist of the part of the antibody's amino acid sequence that binds to the antigen. Due to the compared to the single domain antibodies , scFv fragments , Fab fragments , F (ab) 2 fragments or antibodies, microantibodies have a smaller size and molar mass and are therefore more easily absorbed by cells , e.g. B. seventeen amino acids .

A microantibody was developed to inhibit the replication of HIV in cell culture based on the CDR-H3 region of the IgG 1 antibody called F58. More microantibody were by phage display for binding to the G-CSF - receptor , the protein kinase Aurora-A or to the ganglioside GM1 developed.

Individual evidence

  1. ↑ a b N. A. Jackson, M. Levi, B. Wahren, NJ Dimmock: Properties and mechanism of action of a 17 amino acid, V3 loop-specific microantibody that binds to and neutralizes human immunodeficiency virus type 1 virions. In: The Journal of general virology. Volume 80 (Pt 1), January 1999, pp. 225-236, ISSN  0022-1317 . PMID 9934706 .
  2. ↑ a b C.J. Heap, Y. Wang, TJ Pinheiro, SA Reading, KR Jennings, NJ Dimmock: Analysis of a 17-amino acid residue, virus-neutralizing microantibody. In: The Journal of general virology. Volume 86, Pt 6 June 2005, pp. 1791-1800, ISSN  0022-1317 . doi : 10.1099 / vir.0.80812-0 . PMID 15914858 .
  3. ↑ D. Fujiwara, I. Fujii: Phage selection of peptide "microantibodies". In: Current protocols in chemical biology. Volume 5, Number 3, 2013, pp. 171-194, ISSN  2160-4762 . doi : 10.1002 / 9780470559277.ch130039 . PMID 24391082 .