Pribnow box
The Pribnow-Box , also known as Pribnow-Schaller-Box , is a promoter element found in 1975 by David Pribnow and Heinz Schaller for gene regulation of transcription , i.e. the transcription of a gene from DNA to RNA , in bacteria and bacteriophages . The Pribnow box is a DNA sequence in the promoter region of prokaryotic genes with the consensus sequence 5'-TATAAT-3 ' . This sequence is found about 10 base pairs in front of the transcription start point , which is why this region is also referred to as the −10 region of the promoter. In eukaryotes there is a similar sequence called the TATA box .
Frequency of occurrence of the respective base:
| T | A. | T | A. | A. | T |
| 77% | 76% | 60% | 61% | 56% | 82% |
In 1977 a total of 21 promoter sequences were known for which the above consensus sequence is found. The distance to the start of transcription is five to seven base pairs.
The prokaryotic promoter is also composed of a −35 region (35 base pairs in front of the transcription start point) and possibly other regulatory elements that are either at position −41 or −61 . The similarity of a promoter to the consensus sequence determines the transcription rate of a gene and thus makes a contribution to the level of expression .
Web links
- Pribnow box, minus 10 box in the lexicon of biology at Spektrum.de
Individual evidence
- ↑ D. Pribnow: Nucleotide Sequence of an RNA Polymerase Binding Site at an Early T7 Promoter. In: Proc. Natl. Acad. Sci. USA , Vol. 72, 1975, No. 3, pp. 784-788. PMID 1093168 , PMC 432404 (free full text)
- ↑ D. Pribnow: Bacteriophage T7 early promoters: nucleotide sequences of two RNA polymerase binding sites. In: J. Mol. Biol. , Vol. 99, 1975, No. 3, pp. 419-443. PMID 765476
- ↑ H. Schaller et al .: Nucleotide Sequence of an RNA Polymerase Binding Site from the DNA of Bacteriophage fd. In: Proc. Natl. Acad. Sci. USA , Vol. 72, 1975, No. 2, pp. 737-741. PMID 1054851 , PMC 432391 (free full text)