TATA box

The TATA box , even Goldberg-Hogness box , is a 1978 Michael L. Goldberg and David Hogness found DNA sequence in the promoter region of a gene , the gene regulation of transcription in eukaryotes . The TATA box can also be found in archaea . In bacteria there is a similar sequence called the pribnow box .

General

In order to read the DNA and generate the RNA (transcription), the part to be converted into RNA must have certain “markings” in front of the coding sequence (in the promoter). Only then can the proteins required for this step bind to the DNA. This is done via the so-called TATA box. The name TATA comes from the abbreviation for the base sequence T (hymin) - A (denin) - T (hymin) - A (denin). A box is a common term in molecular biology for a defined, known area, usually a functional section in the genome.

procedure

The TATA box serves as a starting point or starting point for the assembly of general transcription factors . Transcription factors are used to control transcription. 25 nucleotides downstream is the starting point at which the RNA polymerase begins to transcribe. The sequences of the TATA box (multiple adenine and thymine) are gene regulatory and serve as binding points for gene regulatory proteins, which in turn influence the speed of initiation of the RNA polymerase on the DNA. The regulatory sequences can either be close to the promoter (upstream) or behind the gene (downstream).

construction

The TATA box is a DNA sequence in the promoter region of many eukaryotic genes with the consensus sequence 5'-TATAAA-3 ' . This sequence is usually located 25 to 30 base pairs before the starting point of transcription. The so-called TATA box binding protein (TBP) binds to this sequence and is the only transcription factor that binds to the small groove in the DNA. The binding of the TBP to the TATA box is important for the formation of the transcription initiation complex (transcription factors and RNA polymerase).

Web links

TATA box binding proteins

Individual evidence

^ RP Lifton, ML Goldberg, RW Karp, DS Hogness: The organization of the histone genes in Drosophila melanogaster: functional and evolutionary implications. In: Cold Spring Harb Symp Quant Biol. Volume 42, 1978, pp. 1047-1051. PMID 98262
↑ ST Smale, JT Kadonaga: The RNA polymerase II core promoter. In: Annu Rev Biochem . Volume 72, 2003, pp. 449-479. PMID 12651739 . ( PDF ( Memento of October 31, 2006 in the Internet Archive ))

[1] RP Lifton, ML Goldberg, RW Karp, DS Hogness: The organization of the histone genes in Drosophila melanogaster: functional and evolutionary implications. In: Cold Spring Harb Symp Quant Biol. Volume 42, 1978, pp. 1047-1051. PMID 98262

[2] ST Smale, JT Kadonaga: The RNA polymerase II core promoter. In: Annu Rev Biochem . Volume 72, 2003, pp. 449-479. PMID 12651739 . ( PDF ( Memento of October 31, 2006 in the Internet Archive ))