Pwo polymerase
| DNA polymerase ( Pyrococcus woesei ) | ||
|---|---|---|
| Mass / length primary structure | 779 amino acids | |
| Secondary to quaternary structure | Monomer | |
| Identifier | ||
| Gene name (s) | pole | |
| External IDs |
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| Enzyme classification | ||
| EC, category | 2.7.7.7 , nucleotidyl transferase | |
| Substrate | Deoxyribonucleoside triphosphate + DNA n | |
| Products | Diphosphate + DNA n + 1 | |
The Pwo polymerase is a thermostable DNA polymerase that is used for the polymerase chain reaction . The abbreviation Pwo stands for Pyrococcus woesei , a thermophilic archaebacterium from which this polymerase was isolated. When defective uracil is reached in the DNA, this polymerase breaks off the chain extension and, thanks to this read ahead function, ensures that fewer defective DNA clones are synthesized. It takes much less use than the usual Taq - or Pfu polymerases.
Individual evidence
- ↑ A. Ghasemi, AH Salmanian, N. Sadeghifard, AA Salarian, MK Gholi: Cloning, expression and purification of Pwo polymerase from Pyrococcus woesei. In: Iranian journal of microbiology. Volume 3, Number 3, September 2011, pp. 118-122, ISSN 2008-4447 . PMID 22347593 . PMC 3279813 (free full text).