Swelling reaction

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The swelling reaction is a biochemical method for identifying the glycocalyx of Streptococcus pneumoniae , Klebsiella pneumoniae , Neisseria meningitidis , Haemophilus influenzae , Escherichia coli and Salmonella with the help of antibodies . The swelling reaction is based on the same principle as the Gruber-Widal reaction .

properties

As the antibodies bind to bacterial antigens on the cell wall , the bacterial capsule (glycocalyx) appears cloudy and enlarged under a light microscope . The enlargement of the bacterial capsule was originally referred to as swelling when it was first described by Fred Neufeld in 1902 , although - as is known today - it is not an absorption of liquid. Immune sera were originally used as antibody-containing solutions .

Through the swelling reaction, 93 serotypes of Streptococcus pneumoniae can be distinguished, but PCR-based detection methods are increasingly being used.

literature

  • M. Habib, BD Porter, C. Satzke: Capsular serotyping of Streptococcus pneumoniae using the swelling reaction. In: Journal of visualized experiments: JoVE. Number 84, 2014, p. E51208. doi : 10.3791 / 51208 . PMID 24637727 . PMC 4131683 (free full text).
  • IH Park, DG Pritchard, R. Cartee, A. Brandao, MC Brandileone, MH Took: Discovery of a new capsular serotype (6C) within serogroup 6 of Streptococcus pneumoniae. In: Journal of clinical microbiology. Volume 45, Number 4, April 2007, pp. 1225-1233. doi : 10.1128 / JCM.02199-06 . PMID 17267625 . PMC 1865839 (free full text).
  • S. Oftadeh, C. Satzke, GL Gilbert: Identification of newly described Streptococcus pneumoniae serotype 6D by use of the swelling reaction and PCR. In: Journal of clinical microbiology. Volume 48, Number 9, September 2010, pp. 3378-3379. doi : 10.1128 / JCM.00675-10 . PMID 20610680 . PMC 2937698 (free full text).

Individual evidence

  1. ↑ Fisher, Bruce; Harvey, Richard P .; Champe, Pamela C .: Lippincott's Illustrated Reviews: Microbiology (Lippincott's Illustrated Reviews Series) . Lippincott Williams & Wilkins, Hagerstown, MD, ISBN 0-7817-8215-5 , p. 340.
  2. ^ F. Neufeld: About the agglutination of the pneumococci and about the theory of agglutination. In: Z Hyg Infektionskr. (1902), Volume 40, pp. 54-72.
  3. ↑ Centers for Disease Control and Prevention : PCR Deduction of Pneumococcal Serotypes ( Memento of the original of September 4, 2009 in the Internet Archive ) Info: The archive link was automatically inserted and not yet checked. Please check the original and archive link according to the instructions and then remove this notice. . @1@ 2Template: Webachiv / IABot / www.cdc.gov
  4. ^ R. Pai, RE Gertz, B. Beall: Sequential multiplex PCR approach for determining capsular serotypes of Streptococcus pneumoniae isolates. In: Journal of clinical microbiology. Volume 44, Number 1, January 2006, pp. 124-131. doi : 10.1128 / JCM.44.1.124-131.2006 . PMID 16390959 . PMC 1351965 (free full text).