Guidolin played for the first time in 2003 with the pros on the lower-class ITF Future Tour and ATP Challenger Tour . At the beginning he failed several times in the first rounds, in 2005 he finally managed to get into a final of a future in a double. In the world rankings he was unable to improve in the following years, he only made a breakthrough in 2008 - he won his single and six double titles on the Future Tour - and improved in singles and doubles by a good 500 places each, so that he was in the Top 550 and Top 400 respectively.
A year later, Guidolin celebrated his greatest successes in his career. In May he won his first title on the Challenger Tour. In Blumenau he and Marcelo Demoliner defeated his compatriots Rogério Dutra da Silva and Júlio Silva in three sets in the final . Three months later he repeated this success with Demoliner in Brasília , they beat Ricardo Mello and Caio Zampieri in the final . With further futures titles in doubles, he reached his career record in March 2010 with 167th place. In singles, his greatest success on the Challenger Tour was reaching the semi-finals at the tournament in Belo Horizonte . His best individual placement was a 340th place in May 2011. Two months later, Guidolin ended his professional career.