SEREX

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The acronym SEREX ( engl. Se rological identification of antigens by R ecombinant Ex pression Cloning , dt. Se gical identification of antigens by R ekombinantes Ex pressionsklonieren ) stands for a molecular biological method for identifying antigens . It is used in particular in tumor immunology to identify tumor antigens .

description

The basis of SEREX is that organisms can develop antibodies against autologous tumor antigens from cancer cells . The isolated antibodies can be used in cDNA expression libraries to identify tumor antigens.

The SEREX method can determine the immunogenicity of the tumor antigens, but not the frequency of occurrence and the preferential expression of the protein in the tumor. Over 2000 tumor antigens have been discovered with SEREX to date.

The process was originally developed for tumor immunology in order to generate new targets for cancer immunotherapy , but is now also used in other areas of medicine, such as rheumatology , cardiology and gastroenterology .

The procedure

Schematic cross section through a lambda phage

First, a cDNA library is built up and expressed in Escherichia coli with the help of the bacteriophage lambda expression vector . The lytic plaques formed by E. coli , with the potential antigens, are transferred to membranes . The sera are then incubated with the membranes . Antibodies from the serum that bind to the antigens are then visually detected by anti-human antibodies ( IgG ) conjugated to enzymes .

history

The SEREX process was developed in 1994 by a working group around Michael Pfreundschuh at the Saarland University Hospital .

See also

Individual evidence

  1. ↑ a b S. Mayrhofer: Identification of Hodgkin-associated antigens using SEREX.  ( Page no longer available , search in web archives )  Info: The link was automatically marked as defective. Please check the link according to the instructions and then remove this notice. Dissertation, University of Cologne, 2004@1@ 2Template: Toter Link / kups.ub.uni-koeln.de  
  2. ↑ SY Lee and D. Jeoung: The reverse proteomics for identification of tumor antigens. In: J Microbiol Biotechnol 17, 2007, pp. 879-890. PMID 18050904 (Review)
  3. ^ U. Sahin et al.: Human neoplasms elicit multiplied specific immune responses in the autologous host. In: PNAS 92, 1995, pp. 11810-11813. PMID 8524854

literature

  • M. Pfreundschuh among others: Human tumor antigens recognized by antibodies (SEREX). In: Tumor Antigens Recognized by T Cells and Antibodies. HJ Stauss et al. (Editors), Routledge Chapman & Hall, 2003, ISBN 0-415-29698-6 , pp. 161-171.
  • U. Sahin et al .: Serologically identified tumor antigens as cancer vaccines. In: Cancer vaccines and immunotherapy. PL Stern et al. (Editors), Cambridge University Press, 2000, ISBN 0-521-62263-8 , pp. 195-206.

Review article

  • CG Gunawardana and EP Diamandis: High throughput proteomic strategies for identifying tumor-associated antigens. In: Cancer Lett 249, 2007, pp. 110-119. PMID 17306453
  • D. Jäger: Potential target antigens for immunotherapy identified by serological expression cloning (SEREX). In: Methods Mol Biol 360, 2007, pp. 319-326. PMID 17172736
  • F. Fernández Madrid et al .: Improved approach to identify cancer-associated autoantigens. In: Autoimmun Rev 4, 2005, pp. 230-235. PMID 15893717
  • Y. Wang et al .: Perspectives of SEREX-defined antigens in diagnosis and immunotherapy for gastric cancer. In: Cancer Biol Ther 3, 2004, pp. 806-811. PMID 15467426
  • D. Jäger et al .: Identification of tumor antigens as potential target antigens for immunotherapy by serological expression cloning. In: Cancer Immunol Immunother 53, 2004, pp. 144-147. PMID 14727084
  • G. Li et al .: Identification of tumor antigens by serological analysis of cDNA expression cloning. In: Cancer Immunol Immunother 53, 2004, pp. 139-143. PMID 14722670
  • P. Krebs et al: Autoimmunity seen through the SEREX-scope. In: Autoimmun Rev 2, 2003, pp. 339-345. PMID 14550875
  • YT Chen: Cancer vaccine: identification of human tumor antigens by SEREX. In: Cancer J 6, 2000, pp. 208-217. PMID 10874490
  • O. Türeci et al: Serological analysis of human tumor antigens: molecular definition and implications. In: Mol Med Today 3, 1997, pp. 342-349. PMID 9269687
  • YT Chen: Identification of human tumor antigens by serological expression cloning: an online review on SEREX. In: Cancer Immun 2004 [updated 2004 Mar 10; cited 2004 Apr 1].

Technical article

  • TA Hampton et al .: SEREX analysis for tumor antigen identification in a mouse model of adenocarcinoma. In: Cancer Gene Ther 7, 2000, pp. 446-455. PMID 10766351
  • AO Gure et al .: Serological identification of embryonic neural proteins as highly immunogenic tumor antigens in small cell lung cancer. In: PNAS 97, 2000, pp. 4198-4203. PMID 10760287

Web links