SYPRO Ruby

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Structure of SYPRO Ruby, if known.

SYPRO Ruby is a fluorescent dye from ruthenium - chelate type, of the staining of proteins in the course of protein characterization is used mostly in a Western Blot , in 2D gels or IEF gels .

properties

SYPRO Ruby selectively binds to basic amino acids of proteins (including glycoproteins ) in SDS - micelles , but not to DNA . The mutagenicity , reproductive toxicity, and carcinogenicity of SYPRO Ruby are unknown. The absorption maxima are at λ = 280 nm and 450 nm, the emission is maximum at λ = 610 nm. The sensitivity of SDS-PA gels with SYPRO Ruby is around 1 ng protein, which is roughly comparable to silver staining . Due to the fluorescence, the SYPRO Ruby staining has a larger linear dynamic range when estimating the quantity compared to the silver staining or Coomassie , ie a better correlation between the amount of protein and the signal intensity. The SYPRO Ruby dyeing has a comparatively longer dyeing time of four hours.

Individual evidence

  1. ↑ a b c d Aleksandr Petrovich Demchenko: Advanced Fluorescence Reporters in Chemistry and Biology III: Applications in Sensing and Imaging Volume 3 of Advanced Fluorescence Reporters in Chemistry and Biology . Springer 2011. ISBN 9783642180354 . P. 181ff.
  2. ↑ a b c d e f data sheet SYPRO Ruby Protein Gel Stain from Sigma-Aldrich , accessed on November 28, 2013 ( PDF ).
  3. ↑ K. Berggren, TH Steinberg, WM Lauber, JA Carroll, MF Lopez, E. Chernokalskaya, L. Zieske, Z. Diwu, RP Haugland, WF Patton: A luminescent ruthenium complex for ultrasensitive detection of proteins immobilized on membrane supports. In: Analytical biochemistry. Volume 276, Number 2, December 1999, pp. 129-143, ISSN  0003-2697 . doi : 10.1006 / abio.1999.4364 . PMID 10603235 .
  4. ↑ S. Gallagher, D. Chakavarti: staining proteins in gels. In: Journal of visualized experiments: JoVE. Number 17, 2008, S., ISSN  1940-087X . doi : 10.3791 / 760 . PMID 19066521 . PMC 3253607 (free full text).
  5. ↑ T. Rabilloud, JM Strub, S. Luche, A. van Dorsselaer, J. Lunardi: A comparison between Sypro Ruby and ruthenium II tris (bathophenanthroline disulfonate) as fluorescent stains for protein detection in gels. In: Proteomics. Volume 1, Number 5, May 2001, pp. 699-704, ISSN  1615-9853 . doi : 10.1002 / 1615-9861 (200104) 1: 5 <699 :: AID-PROT699> 3.0.CO; 2-C . PMID 11678039 .
  6. ↑ A. Lamanda, A. Zahn, D. Röder, H. Langen: Improved Ruthenium II tris (bathophenantroline disulfonate) staining and destaining protocol for a better signal-to-background ratio and improved baseline resolution. In: Proteomics. Volume 4, Number 3, March 2004, pp. 599-608, ISSN  1615-9853 . doi : 10.1002 / pmic.200300587 . PMID 14997483 .
  7. ↑ MF Lopez, K. Berggren, E. Chernokalskaya, A. Lazarev, M. Robinson, WF Patton: A comparison of silver stain and SYPRO Ruby Protein Gel Stain with respect to protein detection in two-dimensional gels and identification by peptide mass profiling . In: Electrophoresis. Volume 21, Number 17, November 2000, pp. 3673-3683, ISSN  0173-0835 . doi : 10.1002 / 1522-2683 (200011) 21:17 <3673 :: AID-ELPS3673> 3.0.CO; 2-M . PMID 11271486 .