Sequencer
Automatic sequencers (3730xl DNA Analyzer from Applied Biosystems )
(DNA) sequencers , also DNA sequencers , DNA sequencers or DNA sequencers , are laboratory devices for the automatic evaluation of DNA sequencing . Automatic sequencers either take over the separation and analysis of the fragments resulting from the Sanger dideoxy method or the complete implementation of the next generation sequencing process.
history
The first automated sequencer was developed in 1986 by Leroy Hood and colleagues at the California Institute of Technology (Caltech) and was based on the joint electrophoretic separation of four Sanger sequencing batches, which were carried out with fluorescence- labeled primers in a polyacrylamide column .
literature
- Karin Hollricher: High-performance sequencing . In: Laborjournal . No. 4 , 2009, p. 44-48 .
Individual evidence
- ↑ Lloyd M. Smith, Jane Z. Sanders, Robert J. Kaiser, Peter Hughes, Chris Dodd, Charles R. Connell, Cheryl Heiner, Stephen BH Kent, Leroy E. Hood : Fluorescence detection in automated DNA sequence analysis . In: Nature . tape 321 , 1986, pp. 674-679 , doi : 10.1038 / 321674a0 (English).