VSFP

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VSFP ( English Voltage-sensitive fluorescent proteins ) is a group of fusion proteins , which in tension their fluorescence change.

properties

A VSFP consists of two parts. The first part comes from a voltage-dependent protein domain from a phosphatase ( Ci-VSP ) from Cionia intestinalis . The second part consists of a fluorescent protein like the green fluorescent protein (GFP). Different VSFPs were generated with different fluorescent proteins.

Applications

VSFP are used, among other things, as redox-dependent biosensors and as reporter proteins . Alternative optical methods use e.g. B. PROPS or voltage-dependent dyes .

Individual evidence

  1. ↑ H. Mutoh, A. Perron, W. Akemann, Y. Iwamoto, T. Knöpfel: Optogenetic monitoring of membrane potentials. In: Experimental Physiology . Volume 96, Number 1, January 2011, pp. 13-18, ISSN  1469-445X . doi : 10.1113 / expphysiol.2010.053942 . PMID 20851856 .
  2. ↑ H. Mutoh, W. Akemann, T. Knöpfel: Genetically engineered fluorescent voltage reporters. In: ACS Chemical Neuroscience . Volume 3, Number 8, August 2012, pp. 585-592, ISSN  1948-7193 . doi : 10.1021 / cn300041b . PMID 22896802 . PMC 3419450 (free full text).
  3. ↑ Y. Murata, H. Iwasaki, M. Sasaki, K. Inaba, Y. Okamura: Phosphoinositide phosphatase activity coupled to an intrinsic voltage sensor. In: Nature . Volume 435, Number 7046, June 2005, pp. 1239-1243, ISSN  1476-4687 . doi : 10.1038 / nature03650 . PMID 15902207 .
  4. ↑ A. Perron, W. Akemann, H. Mutoh, T. Knöpfel: Genetically encoded probes for optical imaging of brain electrical activity. In: Progress in brain research. Volume 196, 2012, pp. 63-77, ISSN  1875-7855 . doi : 10.1016 / B978-0-444-59426-6.00004-5 . PMID 22341321 .
  5. ↑ JM Kralj, DR Hochbaum, AD Douglass, AE Cohen: Electrical spiking in Escherichia coli probed with a fluorescent voltage-indicating protein. In: Science . Volume 333, Number 6040, July 2011, pp. 345-348, ISSN  1095-9203 . doi : 10.1126 / science.1204763 . PMID 21764748 .