BisTris
| Structural formula | ||||||||||||||||
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| General | ||||||||||||||||
| Surname | BisTris | |||||||||||||||
| other names |
Bis (2-hydroxyethyl) amino-tris (hydroxymethyl) methane |
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| Molecular formula | C 8 H 19 NO 5 | |||||||||||||||
| Brief description |
white solid |
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| properties | ||||||||||||||||
| Molar mass | 209.24 g mol −1 | |||||||||||||||
| Physical state |
firmly |
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| Melting point |
102-103 ° C |
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| safety instructions | ||||||||||||||||
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| As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions . | ||||||||||||||||
BisTris is the abbreviation for bis (2-hydroxyethyl) amino-tris (hydroxymethyl) methane , a buffer substance , with a pK s value of 6.5. The usable pH range thus extends from 5.8 to 7.2.
BisTris is used for BisTris- SDS-PAGE , which is also marketed and patented by Invitrogen under the name NuPAGE . BisTris-containing polyacrylamide gels are not compatible with protein staining with ruthenium complexes.
Individual evidence
- ↑ a b c d e BisTris data sheet at Sigma-Aldrich , accessed on March 13, 2011 ( PDF ).
- ↑ Patent application US20060278531 : Electro-blotting devices, systems, and kits, and methods for their use. Filed August 21, 2006 , published December 14, 2006 , applicant: Life Technologies Corporation, inventor: Margalit et al.
- ↑ J. Moebius, K. Denker, A. Sickmann: Ruthenium (II) tris-bathophenanthroline disulfonate is well suitable for Tris-Glycine PAGE but not for Bis-Tris gels. In: Proteomics. Volume 7, Number 4, February 2007, pp. 524-527, doi : 10.1002 / pmic.200600642 , PMID 17309097 .
See also
Web links
- openwetware.org: Protocol BisTris-SDS-PAGE (engl.)