CEF cells

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CEF cells ( chicken embryo fibroblast ) are a mixture of cell types from embryonated chicken eggs that are used, among other things, to make vaccines , e.g. B. smallpox vaccines , mumps vaccines and TBE vaccines .

properties

CEF cells are primary cells obtained from embryonated chicken eggs. In contrast to cell lines for vaccine production, the supply of embryonated hen's eggs represents a limitation in the scalability of vaccine production. CEF cells are freshly prepared and cultivated in cell culture until shortly before confluence and then infected with the respective virus vaccine strain. After a few days (depending on the virus), the cells are disrupted and the viruses are isolated . The viruses may then be inactivated (depending on the type of vaccine) .

DF1 cells are an immortalized cell line derived from CEF cells.

Individual evidence

  1. ^ Y. Genzel: Designing cell lines for viral vaccine production: Where do we stand? In: Biotechnology journal. Volume 10, number 5, May 2015, pp. 728-740, doi : 10.1002 / biot.201400388 , PMID 25903999 .
  2. ↑ T. Betáková, D. Svetlíková, M. Gocník: Overview of measles and mumps vaccine: origin, present, and future of vaccine production. In: Acta virologica. Volume 57, Number 2, 2013, pp. 91-96, PMID 23600866 .
  3. ↑ RD Hess, F. Weber, K. Watson, S. Schmitt: Regulatory, biosafety and safety challenges for novel cells as substrates for human vaccines. In: Vaccine. Volume 30, Number 17, April 2012, pp. 2715-2727, doi : 10.1016 / j.vaccine.2012.02.015 , PMID 22342707 .