Deoxyribonucleotidyl transferase
| DNA nucleotidyl exotransferase | ||
|---|---|---|
| Identifier | ||
| External IDs |
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| Enzyme classification | ||
| EC, category | 2.7.7.31 , nucleotidyl transferase | |
| Response type | Deoxynucleoside triphosphate + DNA n = diphosphate + DNA n + 1 | |
| Substrate | DNA | |
| Products | DNA | |
The terminal deoxynucleotidyl transferase (TdT) is an enzyme which nucleotides to the 3 'terminal end of DNA added. It is the only DNA polymerase that does not require a template strand, but can use single-stranded DNA as a primer . It is significantly involved in the maturation of stem cells into T lymphocytes or B lymphocytes .
use
The enzyme is used in cell biology in the TUNEL method . The property, nucleotides at the 3 'end is to grow in the molecular biology used to DNAs, particularly oligonucleotides to in vitro Mark . In contrast to marking the 5 'end with polynucleotide kinase , non-radioactive markings are also possible. The generation of homopolymer ends can also be used for annealing and subsequent ligation , as well as for generating a template for the PCR in RACE .
literature
- M. Isobe, K. Huebner, J. Erikson, RC Peterson, FJ Bollum, LM Chang, CM Croce: Chromosome localization of the gene for human terminal deoxynucleotidyltransferase to region 10q23-q25. In: Proc. Natl. Acad. Sci. USA 82 (17), September 1985, pp. 5836-5840. doi: 10.1073 / pnas.82.17.5836 . PMC 390648 (free full text). PMID 3862101 .
- TL Yang-Feng, NR Landau, D. Baltimore, U. Francke: The terminal deoxynucleotidyltransferase gene is located on human chromosome 10 (10q23-q24) and on mouse chromosome 19. In: Cytogenet. Cell Genet. 43 (3-4), 1986, pp. 121-126. doi: 10.1159 / 000132309 . PMID 3467897 .
- Richard Hardy: Chapter 7: B Lymphocyte Development and Biology. In: William Paul: Fundamental Immunology. 6th edition. Lippincott Williams & Wilkins, Philadelphia 2008, ISBN 978-0-7817-6519-0 , pp. 237-269.
- J. Faber, H. Kantarjian, MW Roberts, M. Keating, E. Freireich, M. Albitar: Terminal deoxynucleotidyl transferase-negative acute lymphoblastic leukemia. In: Arch. Pathol. Lab. Med. 124 (1), January 2000, pp. 92-97. PMID 10629138 .
- Anthony SY Leong, Kumarason Cooper, F Joel WM Leong: Manual of diagnostic antibodies for immunohistology. 2nd Edition. Greenwich Medical Media, 2003, ISBN 1-84110-100-1 , pp. 413-414.
Individual evidence
- ↑ Edward A. Motea, Anthony J. Berdis: Terminal Deoxynucleotidyl Transferase: The Story of a Misguided DNA Polymerase . In: Biochimica et Biophysica Acta . tape 1804 , no. 5 , May 2010, ISSN 0006-3002 , p. 1151–1166 , doi : 10.1016 / j.bbapap.2009.06.030 , PMID 19596089 , PMC 2846215 (free full text).