Exotoxin A
| Exotoxin A ( Pseudomonas aeruginosa ) | ||
|---|---|---|
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| Ribbon model according to PDB 1IKQ the chloride ions are shown in blue and the sodium ions in pink |
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| Mass / length primary structure | 613 amino acids | |
| Identifier | ||
| Gene name (s) | toxA (PseudoCAP) | |
| External IDs | ||
| Transporter classification | ||
| TCDB | 1.C.73.1.1 | |
| designation | Pseudomonas exotoxin family | |
| Enzyme classification | ||
| EC, category | 2.4.2 , transferase | |
| Response type | Transfer of an ADP ribosyl residue | |
| Substrate | EF-2 + NAD | |
| Products | defective EF-2 | |
| Occurrence | ||
| Parent taxon | Pseudomonas | |
Exotoxin A is known to be an important, virulent lectin from the bacterium Pseudomonas aeruginosa . The molecular mass is 66.5 kDa . The polypeptide exotoxin A consists of three domains , one of which has ADP-ribosyltransferase activity, while the other two domains bind the toxin to sensitive cells and are responsible for the penetration of the first domain into the cytoplasm through the cell membrane .
The biochemical behavior is identical to the reaction of diphtheria toxin A, ie the elongation factor eEF-2 is bound to an ADP-ribosyl-EF2 complex and is therefore no longer available for protein synthesis .
literature
- SP Yates, PL Taylor, R. Jørgensen et al .: Structure-function analysis of water-soluble inhibitors of the catalytic domain of exotoxin A from Pseudomonas aeruginosa. In: Biochem. J. 385 (Pt 3), February 2005, pp. 667-675. doi: 10.1042 / BJ20041480 . PMC 1134741 (free full text). PMID 15458385 .
- SP Yates, AR Merrill: Elucidation of eukaryotic elongation factor-2 contact sites within the catalytic domain of Pseudomonas aeruginosa exotoxin A. In: Biochem. J. 379 (Pt 3), May 2004, pp. 563-572. doi: 10.1042 / BJ20031731 . PMC 1224111 (free full text). PMID 14733615 .
- P. Hafkemeyer, U. Brinkmann, E. Brinkmann, I. Pastan, HE Blum, TF Baumert: Pseudomonas exotoxin antisense RNA selectively kills hepatitis B virus infected cells. In: World J. Gastroenterol. 14 (18), May 2008, pp. 2810-2817. doi: 10.3748 / year 14.2810 . PMC 2710720 (free full text). PMID 18473403 .
- Daniel Stuckey, Shawn Hingtgen, Nihal Karaka, Benjamin Rich, Khalid Shah: Engineering toxin-resistant therapeutic stem cells to treat brain tumors. In: Stem Cells (AlphaMed Express). 2, 2015, pp. 589-600. doi: 10.1002 / stem.1874 .