Exotoxin A

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Exotoxin A ( Pseudomonas aeruginosa )
Exotoxin A (Pseudomonas aeruginosa)
Ribbon model according to PDB  1IKQ
the chloride ions are shown in blue and the sodium ions in pink

Existing structural data : 1IKP , 1IKQ

Mass / length primary structure 613 amino acids
Gene name (s) toxA (PseudoCAP)
External IDs
Transporter classification
TCDB 1.C.73.1.1
designation Pseudomonas exotoxin family
Enzyme classification
EC, category 2.4.2 transferase
Response type Transfer of an ADP ribosyl residue
Substrate EF-2 + NAD
Products defective EF-2
Parent taxon Pseudomonas

Exotoxin A is known to be an important, virulent lectin from the bacterium Pseudomonas aeruginosa . The molecular mass is 66.5  kDa . The polypeptide exotoxin A consists of three domains , one of which has ADP-ribosyltransferase activity, while the other two domains bind the toxin to sensitive cells and are responsible for the penetration of the first domain into the cytoplasm through the cell membrane .

The biochemical behavior is identical to the reaction of diphtheria toxin A, ie the elongation factor eEF-2 is bound to an ADP-ribosyl-EF2 complex and is therefore no longer available for protein synthesis .


  • SP Yates, PL Taylor, R. Jørgensen et al .: Structure-function analysis of water-soluble inhibitors of the catalytic domain of exotoxin A from Pseudomonas aeruginosa. In: Biochem. J. 385 (Pt 3), February 2005, pp. 667-675. doi: 10.1042 / BJ20041480 . PMC 1134741 (free full text). PMID 15458385 .
  • SP Yates, AR Merrill: Elucidation of eukaryotic elongation factor-2 contact sites within the catalytic domain of Pseudomonas aeruginosa exotoxin A. In: Biochem. J. 379 (Pt 3), May 2004, pp. 563-572. doi: 10.1042 / BJ20031731 . PMC 1224111 (free full text). PMID 14733615 .
  • P. Hafkemeyer, U. Brinkmann, E. Brinkmann, I. Pastan, HE Blum, TF Baumert: Pseudomonas exotoxin antisense RNA selectively kills hepatitis B virus infected cells. In: World J. Gastroenterol. 14 (18), May 2008, pp. 2810-2817. doi: 10.3748 / year 14.2810 . PMC 2710720 (free full text). PMID 18473403 .
  • Daniel Stuckey, Shawn Hingtgen, Nihal Karaka, Benjamin Rich, Khalid Shah: Engineering toxin-resistant therapeutic stem cells to treat brain tumors. In: Stem Cells (AlphaMed Express). 2, 2015, pp. 589-600. doi: 10.1002 / stem.1874 .

Individual evidence

  1. Homologues at OMA