Gimenez stain

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Gimenez staining of Rickettsia rickettsii in the hemolymph of ticks

The Gimenez stain is a histological technique for staining bacteria in animal tissues , particularly rickettsia , Legionella , Bartonella and Coxiella burnetii .

properties

The Gimenez stain is used to detect bacterial infections . The staining solution contains basic fuchsin in an aqueous solution with phenol and ethanol (then referred to as carbol fuchsin ). The fuchsin stains Gram-positive and Gram-negative bacteria in red, magenta or pink. A blue-green counterstaining of the fabric is achieved by adding malachite green to the staining solution, alternatively by counterstaining with saffronines . The May-Grünwald staining and, in some cases, the Macchiavello staining (not suitable for staining R. tsutsugamushi ) are used as alternatives to the staining of rickettsiae .

history

Gimenez staining was first described in 1964 by Domingo F. Giménez as a method for staining rickettsiae in yolk sac cultures.

Individual evidence

  1. ↑ a b c D. F. Gimenez: Staining Rickettsiae in Yolk-Sac Cultures. In: Stain technology. Volume 39, May 1964, pp. 135-140, PMID 14157454 .
  2. ^ PW Greer, FW Chandler, MD Hicklin: Rapid demonstration of Legionella pneumophila in unembedded tissue. An adaptation of the Giménez stain. In: American journal of clinical pathology. Volume 73, Number 6, June 1980, pp. 788-790, PMID 6156594 .
  3. ↑ P. Bruneval, J. Choucair, F. Paraf, JP Casalta, D. Raoult, F. Scherchen, JL Mainardi: Detection of fastidious bacteria in cardiac valves in cases of blood culture negative endocarditis. In: Journal of clinical pathology. Volume 54, Number 3, March 2001, pp. 238-240, PMID 11253139 , PMC 1731384 (free full text).
  4. ↑ Richard L. Kradin: Diagnostic Pathology of Infectious Disease eBook. Elsevier Health Sciences, 2010, ISBN 978-1-455-70598-6 , p. 194.
  5. ↑ Lois J. Paradise: Opportunistic Intracellular Bacteria and Immunity. Springer Science & Business Media, 2006, ISBN 978-0-306-46809-4 , p. 142.
  6. ↑ Don J. Brenner: Bergey's Manual of Systematic Bacteriology. Springer Science & Business Media, 2007, ISBN 978-0-387-28022-6 , p. 211.
  7. ^ William Arthur Hagan: Hagan and Bruner's Microbiology and Infectious Diseases of Domestic Animals. Cornell University Press, 1988, ISBN 978-0-801-41896-9 , p. 319.