Safranin T

Structural formula
General
Surname	Safranin T
other names	Safranin O; Safranin A; Safranin Y; 3,7-diamino-2,8-dimethyl-5-phenyl-phenazinium chloride; CI 50240; CI Basic Red 2; Cotton Red; Gossypimin; Tolusafranine; Hidaco Safranin;
Molecular formula	C 20 H 19 ClN 4
Brief description	dark red odorless solid
External identifiers / databases
EC number	207-518-8
ECHA InfoCard	100.006.836
PubChem	2723800
Wikidata	Q413672
properties
Molar mass	350.85 g · mol -1
Physical state	firmly
solubility	moderate in water (50 g l −1 at 20 ° C)
safety instructions
GHS labeling of hazardous substances Caution
H and P phrases	H: 318
	P: 280-305 + 351 + 338-310
	As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions .

Safranin T (Arabic zafaran, yellowness ) is a derivative of phenazine . It is a cationic dye from the Safranine series , with shades between red and purple.

Extraction and presentation

The dye Safranin T is produced synthetically from a mixture of o -toluidine , aniline with hydrochloric acid and sodium nitrite .

use

Safranin T is used for staining purposes in microscopy . It is also used as a dye for leather . In chemical analysis it is also used as a pH and redox indicator . The oxidized form is red, the reduced form is colorless. Safranin T can also be used for Gram staining of bacteria . Furthermore, it is used with astrablau in the double coloring of plant cells , whereby it colors lignified cell walls red, while non- lignified cell walls are colored blue with astra blue. Safranin T is also used for chromaffin staining of epithelial cells of the intestinal mucosa . Mauvein is an analogous dye.

Web links

Chemical Lexicon: Safranin
The Free Dictionary - Medical Dictionary: Safranin O

Individual evidence

↑ ^a ^b ^c ^d Entry on 3,7-diamino-2,8-dimethyl-5-phenylphenazinium chloride in the GESTIS substance database of the IFA , accessed on January 10, 2017(JavaScript required) .
↑ ^a ^b ^c ^d Entry on Safranine. In: Römpp Online . Georg Thieme Verlag, accessed on December 28, 2014.
^ FW Küster, A. Thiel, A. Ruland: Rechentafeln für die Chemische Analytics , p. 79, 105th edition, de Gruyter Verlag, 2002, ISBN 3-11-017566-5 .

[GESTIS-1] Entry on 3,7-diamino-2,8-dimethyl-5-phenylphenazinium chloride in the GESTIS substance database of the IFA , accessed on January 10, 2017(JavaScript required) .

[roempp-2] Entry on Safranine. In: Römpp Online . Georg Thieme Verlag, accessed on December 28, 2014.

[3] FW Küster, A. Thiel, A. Ruland: Rechentafeln für die Chemische Analytics , p. 79, 105th edition, de Gruyter Verlag, 2002, ISBN 3-11-017566-5 .