Glucose oxidase test

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The glucose oxidase test (GOD test) is a chemical detection method for glucose that is used, among other things, as a blood sugar determination method in clinical chemistry for the detection of diabetes mellitus disease . Test kits, for example in the form of test strips, are available commercially.

The GOD test is a composite enzymatic test . The operation of the test is based on the oxidation of glucose by glucose oxidase , an enzyme that the oxygen-dependent oxidation of the C 1 - carbon atom of the sugar catalyzed . This creates gluconolactone and hydrogen peroxide . Gluconic acid is formed from the lactone spontaneously or enzymatically by gluconolactonase . The hydrogen peroxide is then reduced to water in a subsequent color reaction with ABTS . This color reaction is catalyzed by a peroxidase (POD) - usually horseradish peroxidase . This is why we sometimes talk about the “GOD / POD test”. In addition, the hydrogen peroxide formed can be quantified with the help of an electrochemical measurement (see electrochemistry ) . H. an exact determination of the glucose concentration (e.g. in the blood ).

Individual evidence

  1. ↑ D. Keilin, EF Hartree: Specificity of glucose oxidase (notatin). In: The Biochemical journal. Volume 50, Number 3, January 1952, pp. 331-341, PMID 14915954 . PMC 1197657 (free full text).
  2. ↑ SB Bankar, MV Bule, RS Singhal, L. Ananthanarayan: Glucose oxidase - an overview. In: Biotechnology Advances . Volume 27, Number 4, 2009 Jul-Aug, pp. 489-501, doi : 10.1016 / j.biotechadv.2009.04.003 . PMID 19374943 .
  3. ↑ Entry on ABTS. In: Römpp Online . Georg Thieme Verlag, accessed on December 18, 2014.