Hartmut Oschkinat

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Hartmut Oschkinat (2017)

Hartmut Oschkinat (born February 28, 1957 ) is a German structural biologist and professor of chemistry at the Free University of Berlin . He researched biological macromolecules by solid - nuclear magnetic resonance spectroscopy .

He is a member of the editorial board of the Journal of Biomolecular NMR and Structure .

Life and career

Oschkinat studied chemistry at the Johann Wolfgang Goethe University in Frankfurt am Main . He received his doctorate in 1986 under the supervision of his doctoral supervisor Horst Kessler with the thesis "Analysis of the conformation of Cyclosporin in solution using NMR -spectroscopy: development and use of new methods". After a stay as a postdoc with Geoffrey Bodenhausen at the University of Lausanne , Oschkinat went to the Max Planck Institute for Biochemistry in Martinsried in 1987 . There he worked with Marius Clore, Angela Gronenborn and the Nobel Prize winner Robert Huber . After his habilitation at the Technical University of Munich in 1992, Oschkinat worked at the European Molecular Biology Laboratory (EMBL) in Heidelberg. Since 1998 he has been head of the NMR-assisted structural biology department at the Leibniz Institute for Molecular Pharmacology in Berlin.

research

At the beginning of his career, Oschkinat worked in the field of solution NMR and made a decisive contribution to establishing multi-dimensional NMR spectroscopy in structural biology . After applications to determine the three-dimensional structure of soluble proteins such as a Pleckstrin Homology (PH) and WW domain , of which he also characterized protein-protein interactions in more detail and applied fragment-based drug development , Oschkinat shifted his research focus to the investigation of proteins by means of solid - nuclear magnetic resonance spectroscopy with magic angle spinning (MAS) . His research group was the first to determine a protein structure using this method, which is the structure of a microcrystalline sample of an SH3 domain . Since 2005, his group has been studying complex proteins, such as membrane proteins in their native lipid environment , amyloid fibrils and oligomers . In addition, he develops methods to solve particularly challenging structural biological problems, in which solid-state nuclear magnetic resonance spectroscopy can be used with particular benefit. This includes in particular dynamic nuclear polarization and proton detection by means of fast magic angle spinning .

Services

In 1998 Oschkinat was accepted as a member of the European Molecular Biology Organization . From 2009 to 2011 he was Managing Director of the Leibniz Institute for Molecular Pharmacology . From 2012 he was a member of the International Scientific Advisory Board of the Central European Institute of Technology in Brno . In 2013 Oschkinat was accepted as an honorary member of the National Magnetic Resonance Society ( India ). In 2014 Oschkinat received the Günther Laukien Prize , which is awarded annually at the Experimental Nuclear Magnetic Resonance Conference in the USA.

Individual evidence

  1. ^ Symposium: Biological Solid-State NMR and Beyond, in honor of Hartmut Oschkinat's 60th birthday. (jpg) Leibniz Institute for Molecular Pharmacology, accessed on January 24, 2017 .
  2. ^ Staff and Editorial Board of Structure. Retrieved February 14, 2017 .
  3. ^ Editorial board of Journal of Biomolecular NMR. Retrieved February 14, 2017 .
  4. ^ Department of NMR-Supported Structural Biology (Hartmut Oschkinat). Leibniz Institute for Molecular Pharmacology (FMP Berlin), accessed on January 25, 2017 .
  5. ^ H Oschkinat, C. Griesinger, PJ Kraulis, OW Sørensen, RR Ernst, AM Gronenborn, GM Clore: Three-dimensional NMR spectroscopy of a protein in solution . In: Nature . tape 332 , no. 6162 , 1988, pp. 374-376 , doi : 10.1038 / 332374a0 , PMID 3352736 .
  6. ^ MJ Macias, A. Musacchio, H. Ponstingl, M. Nilges, M. Saraste, H. Oschkinat: Structure of the pleckstrin homology domain from beta-spectrin . In: Nature . tape 369 , no. 6482 , 1994, pp. 675-677 , doi : 10.1038 / 369675a0 , PMID 8208297 .
  7. ^ MJ Macias, V. Gervais, C. Civera, H. Oschkinat: Structural analysis of WW domains and design of a WW prototype . In: Nat. Struct. Biol. Band 7 , no. 5 , 2000, pp. 375-379 , doi : 10.1038 / 75144 , PMID 10802733 .
  8. ↑ MJ Macias, M. Hyvönen, E. Baraldi, J. Schultz, M. Sudol, M. Saraste, H. Oschkinat: Structure of the WW domain of a kinase-associated protein complexed with a proline-rich peptide . In: Nature . tape 382 , no. 6592 , 1996, pp. 646-649 , doi : 10.1038 / 382646a0 , PMID 8757138 .
  9. ↑ M. Joshi, C. Vargas, P. Boisguerin, A. Diehl, G. Krause, P. Schmieder, K. Moelling, V. Hagen, M. Schade, H. Oschkinat: Discovery of low-molecular-weight ligands for the AF6 PDZ domain . In: Angew. Chem. Int. Ed. Engl. Volume 45 , no. 23 , 2006, p. 3790-3795 , doi : 10.1002 / anie.200503965 , PMID 16671149 .
  10. ↑ M. Pellecchia, I. Bertini, D. Cowburn, C. Dalvit, E. Giralt, W. Jahnke, TL James, SW Homans, H. Kessler, C. Luchinat, B. Meyer, H. Oschkinat, J. Peng , H. Schwalbe, G. Siegal: Perspectives on NMR in drug discovery: a technique comes of age . In: Nat Rev Drug Discov . tape 7 , no. 9 , 2008, p. 738-745 , doi : 10.1038 / nrd2606 , PMID 19172689 , PMC 2891904 (free full text).
  11. ↑ G. Comellas, CM Rienstra: Protein structure determination by magic-angle spinning solid-state NMR, and insights into the formation, structure, and stability of amyloid fibrils . In: Annu Rev Biophys . tape 42 , 2013, p. 515-536 , doi : 10.1146 / annurev-biophys-083012-130356 , PMID 23527778 .
  12. ^ F. Castellani, B. van Rossum, A. Diehl, M. Schubert, K. Rehbein, H. Oschkinat: Structure of a protein determined by solid-state magic-angle-spinning NMR spectroscopy . In: Nature . tape 420 , no. 6911 , 2002, p. 98-102 , doi : 10.1038 / nature01070 , PMID 12422222 ( nature.com ).
  13. ^ M. Hiller, L. Krabben, KR Vinothkumar, F. Castellani, BJ van Rossum, W. Kühlbrandt, H. Oschkinat: Solid-state magic-angle spinning NMR of outer-membrane protein G from Escherichia coli . In: Chembiochem . tape 6 , no. 9 , 2005, p. 1679-1684 , doi : 10.1002 / cbic.200500132 , PMID 16138308 .
  14. ↑ L. Krabben, BJ van Rossum, S. Jehle, E. Bocharov, EN Lyukmanova, AA Schulga, A. Arseniev, F. Hucho, H. Oschkinat: Loop 3 of short neurotoxin II is an additional interaction site with membrane-bound nicotinic acetylcholine receptor as detected by solid-state NMR spectroscopy . In: J. Mol. Biol. Volume 390 , no. 4 , 2009, p. 662-671 , doi : 10.1016 / j.jmb.2009.05.016 , PMID 19447114 .
  15. ↑ SA Shahid, B. Bardiaux, WT Franks, L. crabs, M. Habeck, BJ van Rossum, D. Left: Membrane protein structure determination by NMR spectroscopy of solid-state microcrystals . In: Nat. Methods . tape 9 , no. 12 , 2012, p. 1212-1217 , doi : 10.1038 / nmeth.2248 , PMID 23142870 .
  16. ↑ N. Ferguson, J. Becker, H. Tidow, S. Tremmel, TD Sharpe, G. Krause, J. Flinders, M. Petrovich, J. Berriman, H. Oschkinat, AR Fersht: General structural motifs of amyloid protofilaments . In: Proc. Natl. Acad. Sci. USA band 103 , no. 44 , 2006, pp. 16248–16253 , doi : 10.1073 / pnas.0607815103 , PMID 17060612 , PMC 1637568 (free full text).
  17. ↑ S. Jehle, P. Rajagopal, B. Bardiaux, S. Markovic, R. Kühne, JR Stout, VA Higman, RE Klevit, BJ van Rossum, H. Oschkinat: Solid-state NMR and SAXS studies provide a structural basis for the activation of alphaB crystallin oligomers . In: Nat. Struct. Mol. Biol. Volume 17 , no. 9 , 2010, p. 1037-1042 , doi : 10.1038 / nsmb.1891 , PMID 20802487 , PMC 2957905 (free full text) - ( nature.com ).
  18. ↑ MA Geiger, M. Orwick-Rydmark, K. Märker, WT Franks, D. Akhmetzyanov, D. Stöppler, M. Zinke, E. Specker, M. Nazaré, A. Diehl, BJ van Rossum, F. Aussenac, T . Prisner, Ü. Akbey, H. Oschkinat: Temperature dependence of cross-effect dynamic nuclear polarization in rotating solids: advantages of elevated temperatures . In: Phys Chem Chem Phys . tape 18 , no. 44 , 2016, p. 30696-30704 , doi : 10.1039 / c6cp06154k , PMID 27791210 .
  19. ↑ D. Stöppler, C. Song, BJ van Rossum, MA Geiger, C. Lang, MA Mroginski, AP Jagtap, ST Sigurdsson, J. Matysik, J. Hughes, H. Oschkinat: Dynamic Nuclear Polarization Provides New Insights into Chromophore Structure in Phytochrome Photoreceptors . In: Angew. Chem. Int. Ed. Engl. Volume 55 , no. 52 , 2016, p. 16017–16020 , doi : 10.1002 / anie.201608119 , PMID 27879035 .
  20. ↑ AJ Nieuwkoop, WT Franks, K. Rehbein, A. Diehl, Ü. Akbey, F. Engelke, L. Emsley, G. Pintacuda, H. Oschkinat: Sensitivity and resolution of proton detected spectra of a deuterated protein at 40 and 60 kHz magic-angle-spinning . In: J. Biomol. NMR . tape 61 , no. 2 , 2015, p. 161-171 , doi : 10.1007 / s10858-015-9904-0 , PMID 25663049 .
  21. ^ Find people in the EMBO Communities. EMBO, accessed January 24, 2017 .
  22. ^ Professor Walter Rosenthal New Scientific Director of the Max Delbrück Center. (No longer available online.) Health Capital Berlin Brandenburg, January 6, 2009, formerly in the original ; accessed on January 24, 2017 (English): "Professor Hartmut Oschkinat shall hold the office of Acting Director of the FMP until a successor has been appointed."
  23. ↑ Volker Haucke is the new director of the FMP. Forschungsverbund Berlin eV, January 9, 2012, accessed on January 24, 2017 : "... says Prof. Hartmut Oschkinat, who has been acting head of the institute for the past three years."
  24. ↑ CEITEC - Step Forward in Material and Life Sciences. (PDF) Technology Center CAS, p. 13 , accessed on January 24, 2017 (English).
  25. ↑ NMRS members. National Magnetic Resonance Society of India, accessed February 27, 2017 .
  26. ↑ Tutorial & Award Lectures. ENC conference, accessed on January 24, 2017 .
  27. ↑ 2014 Laukien: Hartmut Oschkinat (Leibniz Institute, Berlin). Vimeo, accessed January 24, 2017 .
  28. ^ Bruker: Laukien Prize was awarded to six solid-state NMR spectroscopists. Bruker, accessed January 24, 2017 .
  29. ↑ Monday at ENC: Laukien Prize Winners 2014. The Resonance, accessed on January 24, 2017 (English).

Web links

personal data
SURNAME Oschkinat, Hartmut
BRIEF DESCRIPTION German structural biologist and professor of chemistry at the Free University of Berlin
DATE OF BIRTH February 28, 1957