Kinyoun staining

from Wikipedia, the free encyclopedia
Mycobacterial infection of the small intestine , stained according to Kinyoun.

The Kinyoun stain is a histological stain of microorganisms with an acid-fast cell wall , especially mycobacteria , nocardia and some protozoa .

It is named after the first director of the US American hygiene laboratory on Staten Island, Joseph J. Kinyoun (1860-1919).

properties

The Kinyoun stain is similar to the Ziehl-Neelsen stain , but does not require the slides to be heated . The red color is achieved with carbol fuchsin , followed by decolorization in an acidic alcoholic solution and a second coloration with methylene blue . The use of carbol fuchsin in place of basic fuchsin does not require heating. The red color indicates organisms with an acid-fast cell wall, while all other cells are counterstained with methylene blue. As an alternative to one percent sulfuric acid, a three percent hydrochloric acid solution can be used. With sulfuric acid the discoloration is slower, which is why it is used to identify weakly acid-fast microorganisms such as nocardia. As an alternative to methylene blue, brilliant green can be used as a second color. The Kinyoun staining can be combined with the Gram staining ( Gram-Chromotrop-Kinyoun-staining , GCK).

As an alternative to the Kinyoun staining, an auramine - phenol staining can sometimes be carried out.

literature

  • Margaret Bartelt: Diagnostic Bacteriology: A Study Guide ( English ), First. Edition, FA Davis Company, October 4, 1999, ISBN 978-0803603011 , p. 500.

Individual evidence

  1. ↑ D. Zhao, XM Yang, QY Chen, XS Zhang, CJ Guo, XY Che: A modified acid-fast staining method for rapid detection of Mycobacterium tuberculosis. In: Journal of microbiological methods. Volume 91, Number 1, October 2012, pp. 128-132, ISSN  1872-8359 . doi : 10.1016 / j.mimet.2012.07.024 . PMID 22902528 .
  2. ^ FM Salleh, AM Al-Mekhlafi, A. Nordin, '. M. Yasin, HM Al-Mekhlafi, N. Moktar: Evaluation of gram-chromotrope kinyoun staining technique: its effectiveness in detecting microsporidial spores in fecal specimens. In: Diagnostic microbiology and infectious disease. Volume 69, Number 1, January 2011, pp. 82-85, ISSN  1879-0070 . doi : 10.1016 / j.diagmicrobio.2010.08.028 . PMID 21146718 .
  3. ↑ IF Abou El-Naga, MR Gaafar: Auramine-phenol vs. modified Kinyoun's acid-fast stains for detection of coccidia parasites. In: Laboratory medicine. Volume 45, Number 1, 2014, pp. 65-73, ISSN  0007-5027 . PMID 24719990 .