MSR medium

The MSR medium ( acronym for modified Strullu-Romand ) is a nutrient medium that is often used for the in-vitro cultivation of mycorrhizal fungi . It was published in 1996 by S. Declerck et al. developed and enabled the dual cultivation of Glomus versiforme with Ri-T-DNA-transformed carrot roots . The MSR medium is thus similar to the Minimal Medium , which was developed for a similar purpose. The concentration of nutrients was deliberately kept as low as possible in order to enable the germination of the mycorrhizal spores and the simultaneous growth of the symbiosis partner .

By using live plants instead of transformed roots, the development of autotrophic systems is possible. In this case, the MSR medium does not need any sugar, since the mycorrhiza is nourished by the products of photosynthesis .

ingredients

Macronutrients

Magnesium sulfate (MgSO ₄ · 7H ₂ O) 739 mg / l
Potassium nitrate (KNO ₃ ) 76 mg / l
Potassium dihydrogen phosphate (KH ₂ PO ₄ ) 4.1 mg / l
Calcium nitrate (Ca (NO ₃ ) ₂ · 4H ₂ O) 359 mg / l
Potassium chloride (KCl) 65 mg / l

Micronutrients

NaFeEDTA 8 mg / l
Manganese (II) sulfate (MnSO ₄ · 4H ₂ 0) 2.45 mg / l
Zinc sulfate (ZnSO ₄ · 7H ₂ O) 0.29 mg / l
Boric acid (H ₃ Bo ₃ ) 1.86 mg / l
Copper sulfate (CuSO ₄ · 5H ₂ O) 0.24 mg / l
Sodium molybdate (Na ₂ MoO ₄ · 2H ₂ O) 0.0024 mg / l
Ammonium heptamolybdate ((NH ₄ ) ₆ Mo ₇ 0 ₂₄ · 4H ₂ O) 0.035 mg / l

Organic additives

Thiamine 1 mg / l
Pyridoxine 0.9 mg / l
Nicotinic acid 1 mg / l
Pantothenic acid 0.9 mg / l
Cyanocobalamin 0.4 mg / l
Biotin 0.0009 mg / l
Sucrose 10,000 mg / l
Agar 8,000 mg / l

pH before autoclaving: 5.5

Individual evidence

↑ ^a ^b S. Declerck, DG Strullu, C. Plenchettez: In vitro mass-production of the arbuscular mycorrhizal fungus, Glomus versiforme, associated with Ri T-DNA Transformed carrot roots . In: Mycological research , No. 100 (1996), pp. 1237-1242, doi : 10.1016 / S0953-7562 (96) 80186-9 .
↑ L. Voets HD de Boulois L. Renard, D.-G. Strullu, S. Declerck: Development of an autotrophic culture system for the in vitro mycorrhization of potato plantlets . In: FEMS Microbiology Letters , Heft 248 (2005), pp. 111-118, doi : 10.1016 / j.femsle.2005.05.025 .

[a-1] S. Declerck, DG Strullu, C. Plenchettez: In vitro mass-production of the arbuscular mycorrhizal fungus, Glomus versiforme, associated with Ri T-DNA Transformed carrot roots . In: Mycological research , No. 100 (1996), pp. 1237-1242, doi : 10.1016 / S0953-7562 (96) 80186-9 .

[b-2] L. Voets HD de Boulois L. Renard, D.-G. Strullu, S. Declerck: Development of an autotrophic culture system for the in vitro mycorrhization of potato plantlets . In: FEMS Microbiology Letters , Heft 248 (2005), pp. 111-118, doi : 10.1016 / j.femsle.2005.05.025 .