Minimal medium
Also minimal medium to clarify the term
The minimal medium - also M medium or MM - is a nutrient medium that is mostly used in laboratories to study the interactions between plants and mycorrhizal fungi . It was developed by G. Becard and JA Fortin to analyze the exact processes after the beginning of mycorrhization of a carrot root with the fungus Glomus . It is also widely used in tissue culture experiments where nutrient poor media are required.
In microbiology , minimal media are generally used to find out what nutritional requirements a microorganism has during growth.
Most nutrient media, such as the commonly used MS medium , offer sufficient nutrients for healthy and rapid growth of the plants, but the high concentration of sucrose , phosphate and other ingredients prevent the fungal spores from germinating. A dual culture of plant and fungus and the investigation of their interrelationships is therefore not possible. Becard and Fortin have therefore developed a nutrient medium for their experiments that provides just enough nutrients to keep the plants or plant organs alive, while the fungal spores can still germinate and form a mycorrhizal connection with the host .
ingredients
Macronutrients
- Magnesium sulfate (MgSO 4 · 7H 2 O) 731 mg / l
- Potassium nitrate (KNO 3 ) 80 mg / l
- Potassium chloride (KCl) 65 mg / l
- Potassium dihydrogen phosphate (KH 2 PO 4 ) 4.8 mg / l
- Calcium nitrate (Ca (NO 3 ) 2 · 4H 2 O) 288 mg / l
Micronutrients
- NaFeEDTA 8 mg / l
- Potassium iodide (KI) 0.75 mg / l
- Manganese chloride (MnCl 2 · 4H 2 O) 6 mg / l
- Zinc sulfate (ZnSO 4 · 7H 2 O) 2.65 mg / l
- Boric acid (H 3 Bo 3 ) 1.5 mg / l
- Copper sulfate (CuSO 4 · 5H 2 O) 0.13 mg / l
- Sodium molybdate (Na 2 MoO 4 · 2H 2 O) 0.0024 mg / l
Organic additives
- Glycine 3 mg / l
- Thiamine 0.1 mg / l
- Pyridoxine 0.1 mg / l
- Nicotinic acid 0.5 mg / l
- Inositol 50 mg / l
- Agar 10,000 mg / l
- Sucrose 10,000 mg / l
Optimal pH: 5.5
Individual evidence
- ^ Hans G. Schlegel: Allgemeine Mikrobiologie , Thieme, Stuttgart - New York, 1981, p. 170.
- ↑ G. Becard, JA Fortin: Early events of vesicular-arbuscular mycorrhiza formation on Ri T-DNA Transformed roots . In: New Phytologist , No. 108 (1988), pp. 211-218, doi : 10.1111 / j.1469-8137.1988.tb03698.x .