Markus Sauer

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Markus Sauer (born May 6, 1965 in Pforzheim ) is a German biophysicist . He is Professor of Biotechnology and Biophysics at the Biozentrum of the Julius Maximilians University (JMU) Würzburg . Markus Sauer is also a professor at the Rudolf Virchow Center for Integrative and Translational Bioimaging at JMU Würzburg. His research focuses on the development of highly sensitive super-resolution microscopy methods and their application in biomedical issues.

Life

Markus Sauer attended the Hilda-Gymnasium in Pforzheim. After graduating from high school, he studied chemistry at the Technical University of Karlsruhe (1985), Saarland University (1985-1987) and Heidelberg University (1987-1991). He completed his studies in 1991 with a diploma in chemistry. Sauer then worked at the Physico-Chemical Institute of Heidelberg University in the department of Professor Jürgen Wolfrum on the development of a new time-resolved method for DNA sequencing . He received his doctorate in 1995 from Heidelberg University with the dissertation: Development of efficient fluorescent dyes for the highly sensitive detection of biomolecules using time-resolved LIF spectroscopy .

After receiving his doctorate Sauer worked from 1995-1997 as a postdoctoral researcher at the Physics Institute of the University of Heidelberg before there from 1997 to 2003 his own group in the field of single molecule fluorescence spectroscopy using the BioFuture price of the Federal Ministry of Education and Science (BMBF) built . Sauer spent part of his time at the Lawrence Berkeley National Laboratory in Berkeley in the group of Shimon Weiss. On June 12 , 2002 he completed his habilitation in physical chemistry at the University of Heidelberg.

From 2003-2009 Markus Sauer was professor for applied laser physics and laser spectroscopy at Bielefeld University . In 2009 he accepted a professorship for biotechnology and biophysics at the Biozentrum of the JMU Würzburg. 2017-2019 Sauer was Dean of the Faculty of Biology . He has been a member of the Senate of JMU Würzburg since 2019.

Scientific work

In 1995-2000 Markus Sauer developed time and spectrally resolved methods of single molecule detection to study photophysical processes in dyes and multichromophore systems as well as to characterize and identify biomolecules and to determine optical distances far below the diffraction limit . Building on this work, in 2008 he developed the super-resolution microscopy method direct STochastic Optical Reconstruction Microscopy ( d STORM), which for the first time allowed commercially available fluorescent markers such as antibodies for quantitative super-resolution imaging with 20 nm lateral resolution to be used in fixed and living cells. For several years he has been working on the question of how super-resolution microscopy can be used for the early detection and better treatment of diseases.

Publications (selection)

  • Heilemann, M., van de Linde, S., Schüttpelz, M., Kasper, R., Seefeldt, B., Mukherjee, A., Tinnefeld, P., Sauer, M. (2008) Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes Angew. Chem. Int. Ed. 47: 6172-6176.
  • Wombacher, R., Heidbreder, M., van de Linde, S., Sheetz, MP, Heilemann, M., Cornish, V, W ,, Sauer M. (2010) Live-cell super-resolution imaging with trimethoprim conjugates Nat . Methods 7: 717-719.
  • van de Linde, S., Löschberger, A., Klein, T., Heidbreder, M., Wolter, S., Heilemann, M., Sauer, M. (2011) Direct stochastic optical reconstruction microscopy with standard fluorescent probes Nat. Protocols 6: 991-1009.
  • Wolter, S., Löschberger, A., Holm, T., Aufmkolk, S., Dabauvalle, MC, van de Linde, S., Sauer, M. (2012) rapi d STORM: Accurate, fast and open-source software for localization microscopy. Nat. Methods 9: 1040-1041.
  • Niehörster, T., Löschberger, A., Gregor, I., Krämer, B., Patting, M., Koberling, F., Enderlein, J., Sauer, M. (2016) Multi-target spectrally resolved fluorescence lifetime imaging microscopy. Nat. Methods 13: 257-262.
  • Nerreter, T., Letschert, S., Doose, S., Danhof, S., Einsele, H., Sauer, M., Hudecek, M. (2019) Super-resolution microscopy reveals ultra-low CD19 expression on myeloma cells that triggers elimination by CD19 CAR-T, Nat Communications 10: 3137.
  • Wäldchen, F., Schlegel, J., Doose, S., Sauer, M. (2020) Whole-cell imaging of plasma membrane receptors by 3D lattice light-sheet d STORM. Nat Communications 11: 1-6.
  • Zwettler, FU, Spindler, MC, Reinhard, S., Klein, T., Kurz, A., Benavente, R., Sauer, M. (2020) Tracking down the molecular architecture of the synaptonemal complex by expansion microscopy. Nat Communications 11: 3222.
  • Zwettler, FU, Reinhardt, S., Gambarotto, D., Bell, TDM, Hamel, V., Guichard, P., Sauer, M. (2020) Molecular resolution imaging by post-labeling expansion single-molecule localization microscopy (Ex -SMLM). Nat Communications 11: 3388.

Honors and prizes

  • 1998: BioFuture Prize from the BMBF
  • 2012: Most cited article award of the European Photochemistry and Photobiology Society
  • 2016: Gregorio Weber Award for Excellence in Fluorescence and Theory & Applications

Web links

https://www.super-resolution.de/

Individual evidence

  1. ↑ Home - Chair for Biotechnology and Biophysics. Retrieved July 24, 2020 .
  2. ↑ Shimon Weiss - Molecular Biology Institute. Retrieved July 24, 2020 (American English).
  3. ↑ Uni-Intern. (PDF) Retrieved July 24, 2020 .
  4. ^ Senate - University of Würzburg. Retrieved July 24, 2020 .
  5. Jump up ↑ Philip Tinnefeld, Volker Buschmann, Dirk-Peter Herten, Kyung-Tae Han, Markus Sauer: Confocal Fluorescence Lifetime Imaging Microscopy (FLIM) at the Single Molecule Level . In: Single Molecules . tape 1 , no. 3 , 2000, ISSN  1438-5171 , pp. 215-223 , doi : 10.1002 / 1438-5171 (200009) 1: 33.0.CO; 2-S .
  6. ↑ M. Sauer, B. Angerer, W. Ankenbauer, Z. Földes-Papp, F. Göbel: Single molecule DNA sequencing in submicrometer channels: state of the art and future prospects . In: Journal of Biotechnology . tape 86 , no. 3 , April 2001, p. 181-201 , doi : 10.1016 / S0168-1656 (00) 00413-2 .
  7. ↑ Mike Heilemann, Dirk P. Herten, Rainer Heintzmann, Christoph Cremer, Christian Müller: High-Resolution Colocalization of Single Dye Molecules by Fluorescence Lifetime Imaging Microscopy . In: Analytical Chemistry . tape 74 , no. July 14 , 2002, ISSN  0003-2700 , p. 3511-3517 , doi : 10.1021 / ac025576g .
  8. ^ Philip Tinnefeld, Kenneth D. Weston, Tom Vosch, Mircea Cotlet, Tanja Weil: Antibunching in the Emission of a Single Tetrachromophoric Dendritic System . In: Journal of the American Chemical Society . tape 124 , no. 48 , December 2002, ISSN  0002-7863 , p. 14310-14311 , doi : 10.1021 / ja027343c .
  9. ↑ Thomas Heinlein, Andreas Biebricher, Pia Schlüter, Christian Michael Roth, Dirk-Peter Herten: High-Resolution Colocalization of Single Molecules within the Resolution Gap of Far-Field Microscopy . In: ChemPhysChem . tape 6 , no. 5 , May 13, 2005, p. 949-955 , doi : 10.1002 / cphc.200400622 .
  10. ↑ Mike Heilemann, Emmanuel Margeat, Robert Kasper, Markus Sauer, Philip Tinnefeld: Carbocyanine Dyes as Efficient Reversible Single-Molecule Optical Switch . In: Journal of the American Chemical Society . tape 127 , no. 11 , March 2005, ISSN  0002-7863 , p. 3801-3806 , doi : 10.1021 / ja044686x .
  11. ↑ Mike Heilemann, Sebastian van de Linde, Mark Schüttpelz, Robert Kasper, Britta Seefeldt: Subdiffraction-Resolution Fluorescence Imaging with Conventional Fluorescent Probes . In: Angewandte Chemie International Edition . tape 47 , no. 33 , August 4, 2008, p. 6172-6176 , doi : 10.1002 / anie.200802376 .
  12. ↑ Thomas Nerreter, Sebastian Letschert, Ralph Götz, Sören Doose, Sophia Danhof: Super-resolution microscopy reveals ultra-low CD19 expression on myeloma cells that triggers elimination by CD19 CAR-T . In: Nature Communications . tape 10 , no. 1 , July 17, 2019, ISSN  2041-1723 , p. 1–11 , doi : 10.1038 / s41467-019-10948-w , PMID 31316055 .
  13. ↑ Estefanía García-Guerrero, Ralph Götz, Sören Doose, Markus Sauer, Alfonso Rodríguez-Gil: Upregulation of CD38 expression on multiple myeloma cells by novel HDAC6 inhibitors is a class effect and augments the efficacy of daratumumab . In: Leukemia . April 29, 2020, ISSN  0887-6924 , doi : 10.1038 / s41375-020-0840-y .
  14. ↑ Dina Kouhestani, Maria Geis, Saed Alsouri, Thomas GP Bumm, Hermann Einsele: Variant signaling topology at the cancer cell – T-cell interface induced by a two-component T-cell engager . In: Cellular & Molecular Immunology . July 24, 2020, ISSN  1672-7681 , doi : 10.1038 / s41423-020-0507-7 .
  15. ↑ Jan Schlegel, Simon Peters, Sören Doose, Alexandra Schubert-Unkmeir, Markus Sauer: Super-Resolution Microscopy Reveals Local Accumulation of Plasma Membrane Gangliosides at Neisseria meningitidis Invasion Sites . In: Frontiers in Cell and Developmental Biology . tape 7 , September 13, 2019, ISSN  2296-634X , p. 194 , doi : 10.3389 / fcell.2019.00194 , PMID 31572726 .
  16. ↑ Home Page | European Society for Photobiology. Retrieved July 24, 2020 .
  17. ^ Gregorio Weber Award | ISS. Retrieved July 24, 2020 .
personal data
SURNAME Sour, Markus
BRIEF DESCRIPTION German biophysicist
DATE OF BIRTH May 6, 1965
PLACE OF BIRTH Pforzheim