Neisser staining
The Neisser stain is a microbiological stain to show the polar bodies ( metachromatic granules , polar grains , polyphosphate granules) in the cytoplasm of some gram-positive bacteria. The Neisser stain plays a role primarily in the diagnosis of Corynebacterium diphtheriae . The polar bodies appear blue-black after this coloration.
method
The heat-set preparation is covered for two minutes with a freshly prepared solution of one volume of an aqueous-alcoholic crystal violet solution and two volumes of an aqueous iron - methylene blue solution. The counterstaining takes place for one minute with an aqueous chrysoidin solution.
The polar bodies are blue-black, while the rest of the bacterium is yellow-brown.
Historical
The staining technique was developed by the bacteriologist Max Neisser ( 1869 - 1938 , Frankfurt am Main ).
Web links
- Lexicon of biology: Neisser staining