Schäffer reaction

The Schäffer reaction or Schäffersche crossing reaction is a color reaction used in mycology that is used to determine mushroom species . The reaction was found by the German mycologist Julius Schäffer and published in 1933. Using pure aniline or aniline water (a saturated solution of aniline in water), a pipette or a glass rod is used to draw a 10–20 mm long line on the skin of the fungus to be examined, and a line with concentrated nitric acid is drawn crosswise over it . In the case of a positive reaction, the crossing point turns orange-yellow or deep orange to fire red. The test can also be carried out on desiccates . Because of the toxicity and carcinogenicity of aniline, more recent literature advises not to use the Schäffer reaction.

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• Heinrich Dörfelt , Gottfried Jetschke (Ed.): Dictionary of mycology. 2nd Edition. Spectrum Academic Publishing House, Heidelberg / Berlin 2001, ISBN 3-8274-0920-9 .
• German Josef Krieglsteiner , Andreas Gminder (Hrsg.): Die Großpilze Baden-Württemberg . Volume 5: Mushrooms. Agarics III. Ulmer, Stuttgart 2010, ISBN 978-3-8001-3572-1 .
• Josef Breitenbach, Fred Kränzlin (Ed.): Mushrooms of Switzerland. Contribution to knowledge of the fungal flora in Switzerland. Volume 4: Agarics. Part 2: Entolomataceae, Pluteaceae, Amanitaceae, Agaricaceae, Coprinaceae, Bolbitiaceae, Strophariaceae. Mykologia, Luzern 1995, ISBN 3-85604-040-4 .