Potato spindle tuber viroid

Potato spindle tuber viroid
Systematics
Classification : Viroids Family : Pospiviroidae Genre : Pospiviroid Type : Potato spindle tuber viroid
Taxonomic characteristics
Genome : ssRNA circular Symmetry : no capsid Cover : no shell
Scientific name
Potato spindle tuber viroid
Short name
PSTVd
Left
NCBI  Taxonomy: 12892 NCBI  Reference: V01465 ICTV Taxon History: 201854527 ICTVdB Virus Code : 80.001.0.01.001

The Potato Spindle Tuber Viroid (PSTVd, rarely referred to as the potato spindle tuber viroid ) is a pathogen in plants and is one of the viroids . As such, the pathogen has neither a shell nor protein structures, but only consists of a naked molecule of RNA that is resistant to RNases that are present everywhere due to specific folding and formation of hairpin structures .

Act

The PSTVd ​​attacks potato plants and causes them the plant disease "spindle tuber addiction", a typical spindle-shaped growth of the potato tubers caused by the disease. The mechanisms of infectivity and pathogenicity are not yet known. The PSTVd ​​was discovered and characterized in 1971 by Theodor O. Diener at the Plant Virology Laboratory, United States Department of Agriculture, Beltsville, Maryland.

Genome sequence of the PSTVd

The PSTVd ​​consists of 359 ribonucleotides and has the following sequence:

1 CGGAACUAAA CUCGUGGUUC CUGUGGUUCA CACCUGACCU CCUGAGCAGA AAAGAAAAAA 61 GAAGGCGGCU CGGAGGAGCG CUUCAGGGAU CCCCGGGGAA ACCUGGAGCG AACUGGCAAA 121 AAAGGACGGU GGGGAGUGCC CAGCGGCCGA CAGGAGUAAU UCCCGCCGAA ACAGGGUUUU 181 CACCCUUCCU UUCUUCGGGU GUCCUUCCUC GCGCCCGCAG GACCACCCCU CGCCCCCUUU 241 GCGCUGUCGC UUCGGCUACU ACCCGGUGGA AACAACUGAA GCUCCCGAGA ACCGCUUUUU 301 CUCUAUCUUA CUUGCUUCGG GGCGAGGGUG UUUAGCCCUU GGAACCGCAG UUGGUUCCU

Secondary structure of the PSTVd ​​RNA

literature

• Stefan Andreas Gräf: Formation of metastable RNA structures by sequential folding of viroid transcripts . Düsseldorf 1999, chapter properties of the viroid structure. ( archive.org - diploma thesis, University of Düsseldorf).
• TO Diener: Potato spindle tuber “virus”: IV. A replicating, low molecular weight RNA . In: Virology . tape 45 , no. 2 , 1971, p. 411-428 , doi : 10.1016 / 0042-6822 (71) 90342-4 .
• TO Diener, DR Smith: Potato spindle tuber viroid. VI. Monodisperse distribution after electrophoresis in 20 per cent polyacrylamide gels. In: Virology. Volume 46. No. 2, 1971, pp. 498-499, doi: 10.1016 / 0042-6822 (71) 90342-4 , PMID 5130125 .
• TO Diener, DR Smith, MJ O'Brien: Potato spindle tuber viroid. VII. Susceptibility of several solanaceous plant species to infection with low molecular-weight RNA. In: Virology. Volume 48, No. 3, 1972, pp. 844-846, doi: 10.1016 / 0042-6822 (72) 90166-3 , PMID 5031507 .
• TO Diener: Potato spindle tuber viroid. 8. Correlation of infectivity with a UV-absorbing component and thermal denaturation properties of the RNA. In: Virology. Volume 50, No. 2, 1972, pp. 606-609, doi: 10.1016 / 0042-6822 (72) 90412-6 , PMID 4636118 .

Individual evidence

1. ↑ Detlef Wilkens: Viroide - infectious tiny pieces - a molecule as a pathogen ( memento of February 21, 2008 in the Internet Archive ).