Toluidine blue staining

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Toluidine blue staining in vasculitic peripheral neuropathy .

The toluidine blue staining is a histological technique for staining of acid molecules with toluidine blue , particularly of nucleic acids such as DNA or RNA in tissues .

principle

The toluidine blue stain can be used as a vital stain on living cells . It is mostly used to stain tissues of the oral and pharyngeal mucosa . It is used, among other things, to diagnose oral dysplasia and carcinoma , but it is not very specific, which is why other detection methods are usually used afterwards. Toluidine blue accumulates preferentially in degenerate tissues. In addition to nucleic acids, mucins , amyloids , sulfatides , Corynebacterium diphtheriae , Helicobacter pylori and granules of mast cells and endocrine cells are also stained. The toluidine blue staining can also be used for fast sections and epoxy resin - embedded thin sections .

As an alternative to toluidine blue staining, aceto- orcein staining can be used.

Individual evidence

  1. ^ A b c G. Sridharan, AA Shankar: Toluidine blue: A review of its chemistry and clinical utility. In: Journal of oral and maxillofacial pathology: JOMFP. Volume 16, number 2, May 2012, pp. 251-255, doi : 10.4103 / 0973-029X.99081 . PMID 22923899 . PMC 3424943 (free full text).
  2. ↑ RM Richart: A clinical staining test for in vivo delination of Dysplasia and Carcinoma in Situ. In: Am J Obst Gynecol. (1963), Volume 86, p. 703.
  3. ↑ JB Epstein, C. Scully, J. Spinelli: Toluidine blue and Lugol's iodine application in the assessment of oral malignant disease and lesions at risk of malignancy. In: Journal of oral pathology & medicine: official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology. Volume 21, Number 4, April 1992, pp. 160-163, PMID 1376363 .
  4. ↑ J. Tonzetich: orceine staining and the identification of polytene chromosomes. In: Methods in molecular biology (Clifton, NJ). Volume 247, 2004, pp. 249-256, PMID 14707351 .