Single cell analysis

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The single cell analysis ( English single cell analysis , SCA) referred to in the biochemistry , cell biology , and medicine , the examination of individual cells from or in tissues . Characteristic for the methods used is a comparatively high sensitivity (low detection limit ) or the observation of individual cells in microscopy and flow cytometry .

principle

The tissues of organisms contain different types of cells , some of which are only found in small numbers, e.g. B. some stem cells or metastases in the case of tumor diseases . Single cell analysis is used in microscopy, among other things, to study apoptosis , cell migration , cell division and cell proliferation in a single clone . The patch clamp technique and the lab-on-a-chip are used to study the electrophysiology of individual cells such as neurons . Methods with a low detection limit in the analysis of individual cells are e.g. B. DNA sequencing (partially with genome amplification ), RT-PCR , and mass spectrometry .

The DNA and RNA sequencing of individual cells was named method of the year 2013 by the journal Nature Methods .

Individual evidence

  1. ↑ Dario Anselmetti (Ed.): Single Cell Analysis . John Wiley & Sons, 2009, ISBN 978-3-527-62665-6 ( google.com ).
  2. ^ Jonathan V Sweedler, Edgar A. Arriaga: Single cell analysis . In: Anal Bioanal Chem . tape 387 , 2007, p. 1-2 , doi : 10.1007 / s00216-006-0921-4 .
  3. ↑ M. Wu, AK Singh: Single-cell protein analysis. In: Current Opinion in Biotechnology. Volume 23, Number 1, February 2012, pp. 83-88, ISSN  1879-0429 . doi: 10.1016 / j.copbio.2011.11.023 . PMID 22189001 . PMC 3283030 (free full text).
  4. ^ JP Nolan, D. Condello: Spectral flow cytometry. In: Current protocols in cytometry. Volume 63, No. 1, January 2013, Unit 1.27, ISSN  1934-9300 . doi: 10.1002 / 0471142956.cy0127s63 . PMID 23292705 . PMC 3556726 (free full text).
  5. ↑ SL Spencer, PK Sorger: Measuring and modeling apoptosis in single cells. In: Cell . Volume 144, Number 6, March 2011, pp. 926-939, ISSN  1097-4172 . doi: 10.1016 / j.cell.2011.03.002 . PMID 21414484 . PMC 3087303 (free full text).
  6. ↑ JM Rothenberg, M. Sameni, K. Moin, BF Sloane: Live-cell imaging of tumor proteolysis: impact of cellular and non-cellular microenvironment. In: Biochimica et Biophysica Acta . Volume 1824, Number 1, January 2012, pp. 123-132, ISSN  0006-3002 . doi: 10.1016 / j.bbapap.2011.07.025 . PMID 21854877 . PMC 3232330 (free full text).
  7. ^ Y. Lin, R. Trouillon, G. Safina, AG Ewing: Chemical analysis of single cells. In: Analytical Chemistry . Volume 83, Number 12, June 2011, pp. 4369-4392, ISSN  1520-6882 . doi: 10.1021 / ac2009838 . PMID 21500835 . PMC 3115477 (free full text).
  8. ^ S. Lindström, H. Andersson-Svahn: Overview of single-cell analyzes: microdevices and applications. In: Lab-on-a-Chip . Volume 10, No. 24, Dec 21, 2010, pp. 3363-3372. doi: 10.1039 / c0lc00150c .
  9. ↑ WW Soon, M. Hariharan, MP Snyder: High-throughput sequencing for biology and medicine. In: Molecular systems biology. Volume 9, 2013, p. 640, ISSN  1744-4292 . doi: 10.1038 / msb.2012.61 . PMID 23340846 . PMC 3564260 (free full text).
  10. ^ S. Yilmaz, AK Singh: Single cell genome sequencing. In: Current Opinion in Biotechnology. Volume 23, Number 3, June 2012, pp. 437-443, ISSN  1879-0429 . doi: 10.1016 / j.copbio.2011.11.018 . PMID 22154471 . PMC 3318999 (free full text).
  11. ^ T. Kalisky, P. Blainey, SR Quake: Genomic analysis at the single-cell level. In: Annual Review of Genetics . Volume 45, 2011, pp. 431-445, ISSN  1545-2948 . doi: 10.1146 / annurev-genet-102209-163607 . PMID 21942365 . PMC 3878048 (free full text).
  12. ↑ F. Tang, K. Lao, MA Surani: Development and applications of single-cell transcriptome analysis. In: Nature Methods . Volume 8, Number 4 Suppl, April 2011, pp. S6-S11, ISSN  1548-7105 . doi: 10.1038 / nmeth.1557 . PMID 21451510 . PMC 3408593 (free full text).
  13. ↑ A. Ståhlberg, M. Kubista, P. Aman: Single-cell gene-expression profiling and its potential diagnostic applications. In: Expert Review of Molecular Diagnostics . Volume 11, Number 7, September 2011, pp. 735-740, ISSN  1744-8352 . doi: 10.1586 / erm.11.60 . PMID 21902535 . (PDF)
  14. ↑ EJ Lanni, SS Rubakhin, JV Sweedler: Mass spectrometry imaging and profiling of single cells. In: Journal of proteomics. Volume 75, Number 16, August 2012, pp. 5036-5051, ISSN  1876-7737 . doi: 10.1016 / j.jprot.2012.03.017 . PMID 22498881 . PMC 3419297 (free full text).
  15. ↑ N. Tsuyama, H. Mizuno, T. Masujima: Molecular and functional analysis of cellular phenomena using single-cell mass spectrometry. In: Biological and Pharmaceutical Bulletin . Volume 35, Number 9, 2012, pp. 1425-1431, ISSN  1347-5215 . PMID 22975491 .
  16. ↑ SS Rubakhin, EV Romanova, P. Nemes, JV Sweedler: profiling metabolites and peptides in single cells. In: Nature methods. Volume 8, Number 4 Suppl, April 2011, pp. S20-S29, ISSN  1548-7105 . doi: 10.1038 / nmeth.1549 . PMID 21451513 . PMC 3312877 (free full text).
  17. ↑ Anonymous: Method of the Year 2013. In: Nature Methods. 11, 2013, pp. 1–1, doi: 10.1038 / nmeth.2801 .