Indo-1

Structural formula
General
Surname	Indo-1
other names	2- [4- (bis (carboxymethyl) amino) -3- [2- [2- (bis (carboxymethyl) amino) -5-methylphenoxy] ethoxy] phenyl] -1 H -indole-6-carboxylic acid
Molecular formula	C 32 H 31 N 3 O 12
Brief description	light gray solid
External identifiers / databases
CAS number 96314-96-4 PubChem 105060 ChemSpider 94791 Wikidata Q6025416
properties
Molar mass	502.43 g mol −1
Physical state	firmly
Melting point	> 300 ° C
safety instructions
GHS labeling of hazardous substances no GHS pictograms H and P phrases H: no H-phrases P: no P-phrases
As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions .

Indo-1 is a fluorescent dye that changes its optical properties in the presence of calcium ions and is therefore used as a calcium indicator .

properties

Indo-1 is used analogously to Fura-2 , Furaptra and Calcein . In contrast to Fura-2, Indo-1 has two emission maxima . In calcium-free solution it is 475 nm, while with calcium it is shifted to 400 nm. Indo-1 cannot diffuse into cells . In contrast, the lipophilic ester derivatives (e.g. Indo-1 AM ) can penetrate cells and are converted into Indo-1 by esterases . The absorption maximum is 331 nm and the emission maximum is 398 nm in 0.1 molar TRIS solution with pH 8 and 10 mM Ca ²⁺ . In 0.1 molar potassium chloride solution, the absorption maximum is 349 nm and the emission maximum is 482 nm.

Applications

Indo-1 is used, among other things, in fluorescence microscopy and flow cytometry .

Individual evidence

1. ↑ ^{a b c d} Data sheet Indo 1 at Sigma-Aldrich , accessed on May 14, 2014 ( PDF ).
2. ↑ Grynkiewicz G, Poenie M, Tsien RY: A new generation of Ca2 + indicators with greatly improved fluorescence properties . In: J. Biol. Chem. . 260, No. 6, 1985, pp. 3440-3450. PMID 3838314 .