Indo-1
Structural formula | |||||||||||||
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General | |||||||||||||
Surname | Indo-1 | ||||||||||||
other names |
2- [4- (bis (carboxymethyl) amino) -3- [2- [2- (bis (carboxymethyl) amino) -5-methylphenoxy] ethoxy] phenyl] -1 H -indole-6-carboxylic acid |
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Molecular formula | C 32 H 31 N 3 O 12 | ||||||||||||
Brief description |
light gray solid |
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External identifiers / databases | |||||||||||||
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properties | |||||||||||||
Molar mass | 502.43 g mol −1 | ||||||||||||
Physical state |
firmly |
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Melting point |
> 300 ° C |
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safety instructions | |||||||||||||
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As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions . |
Indo-1 is a fluorescent dye that changes its optical properties in the presence of calcium ions and is therefore used as a calcium indicator .
properties
Indo-1 is used analogously to Fura-2 , Furaptra and Calcein . In contrast to Fura-2, Indo-1 has two emission maxima . In calcium-free solution it is 475 nm, while with calcium it is shifted to 400 nm. Indo-1 cannot diffuse into cells . In contrast, the lipophilic ester derivatives (e.g. Indo-1 AM ) can penetrate cells and are converted into Indo-1 by esterases . The absorption maximum is 331 nm and the emission maximum is 398 nm in 0.1 molar TRIS solution with pH 8 and 10 mM Ca 2+ . In 0.1 molar potassium chloride solution, the absorption maximum is 349 nm and the emission maximum is 482 nm.
Applications
Indo-1 is used, among other things, in fluorescence microscopy and flow cytometry .
Individual evidence
- ↑ a b c d Data sheet Indo 1 at Sigma-Aldrich , accessed on May 14, 2014 ( PDF ).
- ↑ Grynkiewicz G, Poenie M, Tsien RY: A new generation of Ca2 + indicators with greatly improved fluorescence properties . In: J. Biol. Chem. . 260, No. 6, 1985, pp. 3440-3450. PMID 3838314 .