Fura-2

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Structural formula
Structural formula of Fura-2
Fura-2 (free acid)
General
Surname Fura-2
Molecular formula
  • C 29 H 27 N 3 O 14
Brief description

light yellow solid

External identifiers / databases
CAS number
PubChem 57054
ChemSpider 51442
Wikidata Q908761
properties
Molar mass 641.5 g mol −1
Physical state

firmly

solubility

soluble in water and DMSO

safety instructions
GHS labeling of hazardous substances
no GHS pictograms
H and P phrases H: no H-phrases
P: no P-phrases
As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions .

Fura-2 is a fluorescent dye that forms chelate complexes with calcium cations (Ca 2+ ) and is used to determine the intracellular calcium concentration. Fura-2 is available as a pentapotassium or pentasodium salt. With the Fura-2AM (Fura-2-Acetoxymethylester) a membrane-permeable derivative is also known.

properties

The calcium complex can be excited with UV light (340  nm to 380 nm) and fluoresces in the visible range. For the photometric determination of the concentration, the emission is observed at approx. 510 nm. The emission rate at this wavelength is directly proportional to the concentration of the (intracellular) calcium ions.

Other fura dyes

In addition to Fura-2, Fura-1 and Fura-3 are also known:

general structures of Fura-1, Fura-2 and Fura-3
For a General structure R.
1 Fura general.svg Benzoate residue Fura-1.svg
2 Remainder Fura-2.svg
3 Benzonitrile residue Fura-3.svg

Alternatives

Aequorin , Furaptra , Calcein and Indo-1 can be used as alternatives .

Individual evidence

  1. ↑ a b c d data sheet Fura-2 from Sigma-Aldrich , accessed on December 26, 2019 ( PDF ).Template: Sigma-Aldrich / name not given
  2. ↑ External identifiers of or database links to Fura-2, pentapotassium salt: CAS number: 113694-64-7, PubChem : 25195388 , ChemSpider : 21865988 , Wikidata : Q27123161 .
  3. ↑ Data sheet Fura 2 pentapotassium salt, ≥80% from Sigma-Aldrich , accessed on December 26, 2019 ( PDF ).
  4. ↑ Grynkiewicz, G. et al. (1985): A new generation of Ca 2+ indicators with greatly improved fluorescence properties . In: J Biol Chem . 260 (6); 3440-3450; PMID 3838314 ; PDF (free full text access)

Web links