Fura-2AM

Structural formula
General
Surname	Fura-2AM
other names	Fura-2-acetoxymethyl ester
Molecular formula	C 44 H 47 N 3 O 24
Brief description	yellow powder
External identifiers / databases
CAS number 108964-32-5 PubChem 3364574 ChemSpider 2610364 Wikidata Q424893
properties
Molar mass	1001.9 g mol −1
Physical state	firmly
safety instructions
GHS labeling of hazardous substances no GHS pictograms H and P phrases H: no H-phrases P: no P-phrases
As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions .

Fura-2-AM is a membrane-permeable derivative of Fura-2 . Fura-2 was developed in 1985 by Roger Tsien and colleagues and is used as a Ca ²⁺ indicator.

properties

Fura-2 is available as a sodium or potassium salt. By binding to acetoxymethyl ester (AM), Fura-2 can penetrate the cell by passive diffusion . In the cell, endogenous esterases split off the AM, which firstly reactivates the fura and secondly prevents it from leaking out of the cell.

The Ca ²⁺ -induced change in fluorescence is measured at 340 and 380 nm, with Ca ²⁺ saturated fura-2 being excited at 340 nm and free fura-2 being excited at 380 nm. The amount of calcium released is determined using the ratio 340/380 nm, since this ratio already takes into account differences in the distribution of Fura-2 within the cell and differences in cell thickness.

Web links

• Fluorescent Ca2 Indicators
• Ca ²⁺ imaging by Fura-2 ( Memento from April 8, 2010 in the Internet Archive )

Individual evidence

1. ↑ ^{a b} Fura 2-AM data sheet from Sigma-Aldrich , accessed on May 7, 2011 ( PDF ).
2. ↑ Grzegorz Grynkiewicz, Martin Poenie, Roger Y. Tsien: A New Generation of Ca2 + Indicators with Greatly Improved Fluorescence Properties . In: The Journal of Biological Chemistry . tape 260 , no. 6 , 1985, pp. 3440-3450 .