AmbLOXe
| LOXe | ||
|---|---|---|
| Mass / length primary structure | 623 amino acids , 71,632 Da | |
| Identifier | ||
| External IDs | ||
| Orthologue | ||
| Axolotl | human | |
| Entrez | NV | 59344 |
| Ensemble | ENSG00000179148 | |
| UniProt | B5A597 | Q9BYJ1 |
| Refseq (mRNA) | NM_001165960.1 | |
| Refseq (protein) | NP_001159432.1 | |
| Gene locus | ||
| PubMed search | NV |
59344
|
AmbLOXe is an enzyme from the group of lipoxygenases that is formed in the axolotl ( Ambystoma mexicanum ) and is involved in the healing of wounds and the regeneration of severed body parts. The word is made up of three parts: LOX stands for lipoxygenases , Amb for ambystoma and e for the place of formation in the epidermis .
properties
LOXe is formed in the epidermis and in blastemas and binds iron ions . The enzyme is a dioxygenase and thus an oxidoreductase . There is a PLAT domain in the first 71 amino acids , followed by the lipoxygenase domain. AmbLOXe was found in the Mexican tail amphibian axolotl ( Ambystoma mexicanum ) in connection with its particularly extensive ability to regenerate entire limbs and organs within a short time .
Regeneration of body parts
In studies of the mechanism of regeneration of body parts in the Mexican axolotl, the enzyme AmbLOXe was found. If there is an injury, the wound is first enclosed by skin. Then a regeneration blastema forms, a collection of undifferentiated cells, in which AmbLOXe is expressed , especially during the development of new limbs . AmbLOXe is supposed to play an important role in relation to cell migration and cell division and thus significantly accelerate wound healing and regeneration.
Possible application in humans
Some research institutions, such as the "Ambystoma Mexicanum Bioregeneration Center" (AMBC) founded in Lower Saxony in 2010, are concerned with transferring the remarkable ability of axolotl regeneration to mammals and humans. In vitro experiments have shown that mammalian cells into which AmbLOXe was introduced showed faster cell migration and thus closed wounds faster than cells that expressed human lipoxygenases. In experiments in vivo , improved wound healing was also shown when AmbLOXe-expressing cells were used. The epidermal lipoxygenase of the axolotl is being investigated for various clinical applications, for example in scar-free wound healing.
Individual evidence
- ↑ a b B. Menger, PM Vogt, C. Allmeling, C. Radtke, JW Kuhbier, K. Reimers: AmbLOXe – an epidermal lipoxygenase of the Mexican axolotl in the context of amphibian regeneration and its impact on human wound closure in vitro. In: Annals of surgery. Volume 253, Number 2, February 2011, pp. 410-418, doi : 10.1097 / SLA.0b013e318207f39c , PMID 21183847 .
- ↑ a b c Uniprot : Entry B5A597 . Retrieved January 4, 2018.
- ↑ Hannover Medical School: Ambystoma Mexicanum Biogeneration Center. Retrieved December 14, 2017.
- ↑ S. Strauss, A. Stamm, C. Liebsch, I. Pepelanova, T. Scheper, PM Vogt: From Axolotl to AmbLOXe - Transferring amphibian regeneration to mammalian wound healing , De Gruyter , accessed on November 12, 2017.