mRNA capping enzyme
| MRNA capping enzyme | ||
|---|---|---|
| Properties of human protein | ||
| Mass / length primary structure | 597 amino acids | |
| Isoforms | 4th | |
| Identifier | ||
| Gene name | RNGTT | |
| External IDs | ||
| Enzyme classification | ||
| EC, category | 3.1.3.33 , phosphatase | |
| Response type | Cleavage of a phosphoester bond | |
| Substrate | 5'-phosphopolynucleotide + H 2 O | |
| Products | Polynucleotide + phosphate | |
| EC, category | 2.7.7.50 , Transferase | |
| Response type | Transfer of a guanylyl residue | |
| Substrate | GTP + 5'pp mRNA | |
| Products | Diphosphate + G (5 ') ppp mRNA | |
| Occurrence | ||
| Homology family | Capping enzyme | |
| Parent taxon | Eukaryotes | |
| Orthologue | ||
| human | House mouse | |
| Entrez | 8732 | 24018 |
| Ensemble | ENSG00000111880 | ENSMUSG00000028274 |
| UniProt | O60942 | O55236 |
| Refseq (mRNA) | NM_001286426 | NM_001305273 |
| Refseq (protein) | NP_001273355 | NP_001292202 |
| Gene locus | Chr 6: 88.61 - 88.96 Mb | Chr 4: 33.31 - 33.5 Mb |
| PubMed search | 8732 |
24018
|
The mRNA capping enzyme (CE) is one of two enzymes in eukaryotes that provide the mRNA with a protective group of molecules, the so-called 5'-cap structure , during transcription . The CE in particular attaches a guanylyl residue to the mRNA, which is later methylated. This requires two reaction steps, which are catalyzed by two different protein domains on the CE enzyme. So it is a bifunctional enzyme. Four CE isoforms are known in humans , which occur in different tissue types and have partly lost their enzymatic activity.
CE is part of the so-called capping complex , which arises after dissociation of the pre-initiation complex after its separation from the promoter and contains among other things the RNA polymerase II and the two capping enzymes.
See also
further reading
- reactome: mRNA capping - doi : 10.3180 / REACT_1470.1
Individual evidence
- ↑ UniProt O60942
- ↑ Buratowski / reactome: Hydrolysis of the 5'-end of the nascent transcript by the capping enzyme ( page no longer available , search in web archives ) Info: The link was automatically marked as defective. Please check the link according to the instructions and then remove this notice.
- ↑ Buratowski / reactome: Formation of the CE: GMP intermediate complex ( page no longer available , search in web archives ) Info: The link was automatically marked as defective. Please check the link according to the instructions and then remove this notice.
- ↑ Buratowski / reactome: Transfer of GMP from the capping enzyme GT site to 5'-end of mRNA ( page no longer available , search in web archives ) Info: The link was automatically marked as defective. Please check the link according to the instructions and then remove this notice.
