PB1

PB1
other names	Polymerase basic protein 1 (influenza virus A); RNA-directed RNA polymerase catalytic subunit;
	Existing structural data : PDB 2ZNL , PDB 2ZTT , PDB 3A1G
Mass / length primary structure	757 amino acids , 86,576 Da
Identifier
External IDs	UniProt : P03431 ;
Enzyme classification
EC, category	2.7.7.48
	Orthologue

PB1 is one of up to eleven different proteins of the influenza virus A (IAV).

properties

PB1 is an internal protein of the IAV and occurs in the virion . It is one of the three viral proteins of the RNA polymerase complex alongside PA and PB2 . Besides PB2, PB1 is a polymerase protein with a basic isoelectric point , while PA has an acidic isoelectric point. Hence the abbreviation of the name.

Occasionally a non-essential protein PB1-F2 is formed from a second open reading frame . The RNA polymerase complex is in the replication cycle for the replication and transcription of viral RNA in charge. The viral mRNA is transcribed using the unique cap snatching mechanism . The cellular mRNA modified with 5'-methylated cap structures are bound by PB2 to the 7-methylguanosine group and then cut by PA 10 to 13 nucleotides according to the cap structure . The short cap-bearing fragments are bound by PB1 and used in the RNA polymerase complex as primers for the transcription of viral RNA. During replication, PB1 initiates RNA synthesis. PB1 is phosphorylated .

Applications

PB1 is being studied as a target in drug development against IAV.

Web links

MeSH PB1

Individual evidence

↑ MR Hilleman : Realities and enigmas of human viral influenza: pathogenesis, epidemiology and control. In: Vaccine Volume 20, 2002, No. 25-26, pp. 3068-3087, PMID 12163258 .
^ RP Kamal, IV Alymova, IA York: Evolution and Virulence of Influenza A Virus Protein PB1-F2. In: International journal of molecular sciences. Volume 19, number 1, December 2017, p., Doi : 10.3390 / ijms19010096 , PMID 29286299 , PMC 5796046 (free full text).
↑ S. Massari, L. Goracci, J. Desantis, O. Tabarrini: Polymerase Acidic Protein-Basic Protein 1 (PA-PB1) Protein-Protein Interaction as a Target for Next-Generation Anti-Influenza Therapeutics. In: Journal of medicinal chemistry. Volume 59, Number 17, September 2016, pp. 7699-7718, doi : 10.1021 / acs.jmedchem.5b01474 , PMID 27046062 .

[1] MR Hilleman : Realities and enigmas of human viral influenza: pathogenesis, epidemiology and control. In: Vaccine Volume 20, 2002, No. 25-26, pp. 3068-3087, PMID 12163258 .

[2] RP Kamal, IV Alymova, IA York: Evolution and Virulence of Influenza A Virus Protein PB1-F2. In: International journal of molecular sciences. Volume 19, number 1, December 2017, p., Doi : 10.3390 / ijms19010096 , PMID 29286299 , PMC 5796046 (free full text).

[3] S. Massari, L. Goracci, J. Desantis, O. Tabarrini: Polymerase Acidic Protein-Basic Protein 1 (PA-PB1) Protein-Protein Interaction as a Target for Next-Generation Anti-Influenza Therapeutics. In: Journal of medicinal chemistry. Volume 59, Number 17, September 2016, pp. 7699-7718, doi : 10.1021 / acs.jmedchem.5b01474 , PMID 27046062 .