Pappenheim staining
With the Panoptic staining according to Pappenheim (named after Artur Pappenheim , 1870-1916) air-dried blood smears are treated with several color solutions. These solutions contain, among other things, stabilizers ( glycerine ), fixatives ( methanol ), acidic and basic dyes . The Pappenheim staining is a combination of the May-Grünwald staining and the Giemsa staining .
Basic dyes such as methylene blue and brilliant cresyl blue form active dye components with a positive charge in aqueous solution and stain cell components with a negative charge a bluish tinge. Acid dyes such as eosin and acid fuchsin break down in aqueous solution into active dye components with a negative charge and color protein structures with a positive charge. Neutrophils can be stained with both basic and acidic dyes.
The Pappenheim staining is a panoptic / panchromatic staining, since basophilic, neutrophilic and eosinophilic structures are shown.
Result
The various cellular and humoral (liquid) components of the blood can be seen in different colors under the light microscope .
| Blood component | Coloring |
|---|---|
| Erythrocytes | pink |
| Nuclei of leukocytes and nucleated erythrocytes | red-violet |
| Eosinophil granules | brick-red to red-brown |
| Basophilic granules | dark purple to black |
| Neutrophil granules | light purple |
| Cytoplasm of lymphocytes | Light Blue |
| Monocyte plasma | gray-blue |
literature
- Maria Mulisch, Ulrich Welsch (ed.): Romeis - microscopic technology . 19th edition. Springer Spectrum, Berlin, Heidelberg 2015, ISBN 978-3-642-55189-5 .