Phytohemagglutinin is found in various legumes . As lectins, the two proteins of phytohemagglutinin bind oligosaccharides . They belong to the group of hemagglutinins and create clumping of blood cells by binding the oligosaccharides on their cell surface . Phytohemagglutinin is a mitogen and activates T lymphocytes and monocytes . It is therefore used as a positive control in various in-vitro tests for immune reactions , such as in the ELISpot . It is also used for cell cultures of lymphocytes because it stimulates their cell division . It is also used to produce karyograms .
Found in legumes
Phytohemagglutinin is a toxin in legumes and can be denatured and thus inactivated by boiling at 100 ° C for ten minutes . Insufficient heating to 80 ° C can increase the toxicity five times compared to the raw state. The following table shows the lectin content of various legumes (according to the USFDA ):
|variety||Lectin content in hemagglutinating units (HAE)|
|Red kidney beans , raw||20,000-70,000|
|White kidney bean , raw||7,000-23,000|
|Broad bean , raw||1000-7000|
|Red kidney beans, well cooked||200-400|
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