TSE buffer

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The TSE buffer is a buffer that is used in biochemistry to dissolve, purify and store proteins and nucleic acids and to purify cells prior to cell disruption .

properties

The TSE buffer consists of TRIS (50 mM ) to stabilize the pH value , 50 mM sodium chloride for the ionic strength and 5 mM EDTA as a chelator for divalent metal ions such as Mg 2+ . By the binding of Mg 2+ are nucleases and DNA -modifizierende enzymes (eg. B. DNA polymerases ) inhibited. The pH is adjusted to 8 with hydrochloric acid .

Individual evidence

  1. ↑ Gerald Litwack: Receptor Purification. Springer Science & Business Media, 2012, ISBN 978-1-4612-0461-9 , p. 48.
  2. ^ John R. Gosden: Chromosome Analysis Protocols. Springer Science & Business Media, 1994, ISBN 978-1-59259-516-7 , p. 444.
  3. ↑ John R. Stephenson: Diagnostic Virology Protocols. Springer Science & Business Media, 1998, ISBN 978-0-89603-479-2 , p. 206.
  4. ↑ Ivan Lefkovits : Immunological Methods. Elsevier, 2014, ISBN 978-1-4832-6963-4 , p. 5.