Triokinase

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Triokinase
other names
  • DAK
  • Triokinase / FMN cyclase
Properties of human protein
Mass / length primary structure 575 amino acids, 58,947 Da
(isoform 1)
Identifier
Gene name TKFC
External IDs
Enzyme classification
EC, category 2.7.1.28
Orthologue
human House mouse
Entrez 26007 225913
Ensemble ENSG00000149476 ENSMUSG00000034371
UniProt Q3LXA3 Q8VC30
Refseq (mRNA) NM_015533 NM_145496
Refseq (protein) NP_056348 NP_663471
Gene locus Chr 11: 61.33 - 61.35 Mb Chr 19: 10.59-10.61 Mb
PubMed search 26007 225913

Triokinase ( TK , also called triose kinase ) is an enzyme that catalyzes the phosphorylation of dihydroxyacetone and glyceraldehyde . The metabolic products are further processed in glycolysis .

properties

There are three possible ways to further convert D- glyceraldehyde, the phosphorylation of D -glyceraldehyde with the aid of triokinase being the preferred way due to its low K m value (0.01  mM ). About 90% of the D- glyceraldehyde formed from fructose is metabolized in this way. In addition, the triokinase is highly active in the mammalian liver. At a D- glyceraldehyde concentration of 10 mM, the ATP used for phosphorylation is used up in a liver cell .

Reactions

1,3-dihydroxypropan-2-ones 200.svg+ ATP + ADP + H + DHAP struct.svg

1) Dihydroxyacetone is phosphorylated to dihydroxyacetone phosphate with the help of triose kinase while consuming ATP .

Entry of fructose into glycolysis.
2) Triose kinase (TK) phosphorylates D- glyceraldehyde (4) with consumption of ATP to D -glyceraldehyde-3-phosphate (5).

literature

  • Jeremy M. Berg, John L. Tymoczko, Lubert Stryer: Stryer Biochemistry. 7th edition. Springer-Verlag, Berlin / Heidelberg 2014, ISBN 978-3-8274-2988-9 , p. 473.

Individual evidence

  1. Ernest Lindbergh: Hemolytic Anemia in Disorders of Red Cell Metabolism . Springer Science & Business Media, 2012, ISBN 978-1-4684-2457-7 , p. 13 ( limited preview in Google Book search).
  2. D. Burman, JB Holton: Inherited Disorders of Carbohydrate Metabolism: Monograph based upon Proceedings of the Sixteenth Symposium of The Society for the Study of Inborn Errors of Metabolism . Springer Science & Business Media, 2012, ISBN 978-94-009-9215-3 ( limited preview in Google book search).
  3. Peter J. O'Brien, William Robert Bruce: Endogenous Toxins, 2 Volume Set: Targets for Disease Treatment and Prevention . John Wiley & Sons, 2009, ISBN 978-3-527-32363-0 , pp. 189 ( limited preview in Google Book search).