Bacillus sporothermodurans

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Bacillus sporothermodurans
Systematics
Department : Firmicutes
Class : Bacilli
Order : Bacillales
Family : Bacillaceae
Genre : Bacillus
Type : Bacillus sporothermodurans
Scientific name
Bacillus sporothermodurans
Pettersson et al. 1996

Bacillus sporothermodurans is an aerobic gram-positive bacterium . There are two different types of this bacterium, which differ phenotypically and genotypically . Types I, II and III show length variations in the 16S rRNA. However, these variations do not play a role in diagnostic evidence.

Bacillus sporothermodurans cells from culture are typically long (> 30 µm), filamentous rods 0.7 µm in diameter. The cells can only be stained unevenly, grainy or like a string of pearls with Gram staining. The cells are mobile by unilateral flagella . The terminal spores are ellipsoidal and up to 1.7 µm long. The colonies are small, smooth, whitish to beige and do not have a soluble pigment . B. sporothermodurans is strictly aerobic, catalase and oxidase positive .

Bacillus sporothermodurans can be found in ultra-high temperature milk and dairy products . Bacillus sporothermodurans can reach up to 10 5 CFU / ml in UHT milk . The vegetative cells are not pathogenic and do not lead to changes in taste or appearance.

However, their occurrence in UHT milk is undesirable because such products do not comply with the provisions of the European Union . Using MALDI-TOF , after pre-incubation (“stress test”) and several washing steps, a direct detection can be carried out like from a blood culture .

The heat resistance is based on the ability to form spores . The spores can survive the ultra-high temperature particularly well if the heat is indirect and there are fewer chemical changes in the milk. The spores then multiply in the packaged milk after heating is complete.

Individual evidence

  1. ^ A b B. Pettersson, F. Lembke, P. Hammer, E. Stackenbrandt, G. Priest: Bacillus sporothermodurans, a new species producing highly heatresistant endospores. In: Int. J. Syst. Bacteriol. 46. ​​1996, 759-764
  2. ↑ P. Hammer, G. Suhren and W. Heeschen: Pathogenicity testing of unknown mesophilic Bacillus species from UHT milk. In: Bull. Int. Dairy Fed. 302. 1995, 56-57) (L. Herman, M. Heyndrickx and N. Vaerewijck and Klijn: Bacillus sporothermodurans - a Bacillus forming highly heat-resistant spores. 3. Isolation and methods of detection. In: Bull. Int. Dairy Fed. 357.2000 9-14.
  3. ↑ Directive 92/46, Council of the European Communities of June 16. Health rules for the production and the trade of raw milk, heat treated milk, and products based on milk. In Official Journal of the European Community. Number L268, p. 1-32
  4. ↑ Christine Wind, Leonie Böhmer: MALDI-TOF mass spectrometry - a technology conquers microbiological examination
  5. ↑ G. Montanari, A. Borsari, C. Chiavari, C. Ferri, C. Zambonelli, and L. Grazia: Morphological and phenotypical characterization of Bacillus sporothermodurans. J. Appl. Microbiol . 97.. 2004, 802-809