Bouin's solution
The Bouin's solution (also Bouin's solution , Bouin's fluid ) is a solution that is used in histology for fixation .
properties
| safety instructions | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Surname |
|
||||||||
| CAS number |
37330-50-0 |
||||||||
|
|||||||||
Bouin's solution consists of picric acid (saturated solution, 0.04 molar), 5% (V / V) acetic acid (0.9 molar) and 10% (V / V) formaldehyde in aqueous solution. It is used, among other things, to fix biopsies of the gastrointestinal tract and soft tissue , as it then allows a higher-contrast staining of cell nuclei compared to neutrally buffered ten percent formaldehyde solution . The Bouin solution has a pH value between 1.5 and 2. It is less suitable for electron microscope preparations. Fixation with Bouin's solution is used, among other things, before hematoxylin-eosin staining or trichrome staining . Formaldehyde-fixed fabrics are usually stained with Bouin's solution before trichrome staining . The acetic acid it contains lyses erythrocytes , dissolves and chelates iron and calcium ions. Alternatively, formic acid can be used. Formaldehyde creates a basophilic cytosol , but this is compensated for by the picric acid.
Undried tissues should be fixed in Bouin's solution for less than 24 hours. Excess picric acid is washed out with ethanol-water solutions in order to ensure that the preparation can be colored over the long term. Accordingly, Bouin-fixed tissue should be stored in an ethanol-water solution.
With limitations, DNA for a polymerase chain reaction or RNA for RT-PCR can be extracted from Bouin-fixed tissues . For immunostaining one is often in Bouin-fixed tissues because of the contained formaldehyde antigen retrieval necessary.
Modifications
The Gendre solution is an alcoholic variant of Bouin's solution, in which the saturated picric acid solution is made with an alcoholic solution. This means that polysaccharides such as glycogen are retained in the preparation.
The Hollande solution also contains copper (II) acetate , which preserves the cell membrane of erythrocytes and the granules of eosinophils and endocrine cells.
Alternatives
Alternatively, fixation with copper salts according to Hollande or with mercury salts can be carried out instead of the Bouin solution, e.g. B. with B5 or according to Zenker.
history
The Bouin solution was developed by Pol Bouin in 1897 .
Individual evidence
- ↑ Freida L. Carson, Christa Hladik: Histotechnology: A Self-Instructional Text , 3rd Edition, American Society for Clinical Pathology Press, Hong Kong 2009, ISBN 978-0-89189-581-7 , p. 19.
- ^ Roger J. Lincoln, John Gordon Sheals (ed.): Invertebrate Animals - Collection and Preservation , 1st edition, British Museum (Natural History), UK 1979, ISBN 0 521 296773 , p. 128.
- ↑ a b Bouin's solution data sheet at Sigma-Aldrich , accessed on May 25, 2017 ( PDF ).
- ↑ JM French: Imaging and morphology in reproductive toxicology - progress to date and future directions . In: Reproductive Toxicology . tape 48 , September 2014, p. 37-40 , doi : 10.1016 / j.reprotox.2014.03.008 , PMID 24681297 .
- ^ John D. Bancroft, Marilyn Gamble (Eds.): Theory and Practice of Histology Techniques , 6th Edition, Churchill Livingstone Elsevier, China 2008, ISBN 978-0-443-10279-0 , p. 72.
- ^ I. Hostein, I. Soubeyran, H. Eghbali, S. de Resende, M. Longy, P. Soubeyran: Polymerase chain reaction diagnosis of t (14; 18) from paraffin-embedded tissues fixed with Holland Bouin fluid. In: Diagnostic Molecular Pathology Volume 7, Number 3, June 1998, pp. 184-188. PMID 9836076 .
- ↑ A. Gloghini, B. Canal, U. Klein, L. Dal Maso, T. Perin, R. Dalla-Favera, A. Carbone: RT-PCR analysis of RNA Extracted from Bouin-fixed and paraffin-embedded lymphoid tissues. In: The Journal of molecular diagnostics: JMD. Volume 6, Number 4, November 2004, pp. 290-296. doi : 10.1016 / S1525-1578 (10) 60524-7 . PMID 15507667 . PMC 1867484 (free full text).
- ↑ AV Bassarova, AA Popov: Immunohistochemical detection of p53 effect of fixation and methods of antigen retrieval. In: Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society. Volume 36, Number 3, 1998, pp. 127-132. PMID 9773296 .
- ↑ G. Baloglu, A. Haholu, Z. Kucukodaci, I. Yilmaz, S. Yildirim, H. Baloglu: The effects of tissue fixation alternatives on DNA content: a study on normal colon tissue. In: Applied Immunohistochemistry & Molecular Morphology Volume 16, Number 5, October 2008, pp. 485-492. doi : 10.1097 / PAI.0b013e31815dffa6 . PMID 18594471 .
- ^ C. Ortiz-Hidalgo: Pol André Bouin, MD (1870-1962). Bouin's fixative and other contributions to medicine. In: Archives of pathology & laboratory medicine. Volume 116, Number 8, August 1992, pp. 882-884. PMID 1497471 .