Cutinase

Cutinase
	according to PDB 1CEX
	Existing structural data : 1CEX
Mass / length primary structure	230 amino acids
Identifier
External IDs	UniProt : P00590 ;
Enzyme classification
EC, category	3.1.1.74 , carboxyl ester hydrolase
Substrate	Cutin + H 2 O
Products	Cutin monomers

Modeling of genetically modified enzymes (cutinases)

Cutinase or cutin hydrolase is an enzyme that belongs to the α / β hydrolases and hydrolyses cutin .

properties

Cutinase is produced by some phytopathogenic fungi and bacteria , among others . Through the cutinase, the fungi are able to break down the ester bond of the cutin in the cuticula of the plants and thus penetrate the plants.

Like all enzymes , however , they catalyze both forward and reverse reactions , which means that under suitable conditions they are able to synthesize polyesters by polycondensation .

literature

S. Longhi, C. Cambillau: Structure-activity of cutinase, a small lipolytic enzyme. In: Biochimica et Biophysica Acta . Volume 1441, Numbers 2-3, November 1999, pp 185-196, PMID 10570246 .

Individual evidence

↑ JA Sweigard, FG Chumley, B. Valent: Cloning and analysis of CUT1, a cutinase gene from Magnaporthe grisea. In: Molecular & general genetics: MGG. Volume 232, Number 2, March 1992, pp. 174-182, PMID 1557023 .
↑ S. Chen, L. Su, J. Chen, J. Wu: Cutinase: characteristics, preparation, and application. In: Biotechnology Advances . Volume 31, number 8, December 2013, pp. 1754-1767, doi : 10.1016 / j.biotechadv.2013.09.005 . PMID 24055682 .
↑ PE Kolattukudy: polyester in higher plants. In: Advances in Biochemical Engineering / Biotechnology . Volume 71, 2001, pp. 1-49, PMID 11217409 .
^ TF Pio, GA Macedo: Cutinases: properties and industrial applications. In: Advances in applied microbiology. Volume 66, 2009, pp. 77-95, doi : 10.1016 / S0065-2164 (08) 00804-6 . PMID 19203649 .

[1] JA Sweigard, FG Chumley, B. Valent: Cloning and analysis of CUT1, a cutinase gene from Magnaporthe grisea. In: Molecular & general genetics: MGG. Volume 232, Number 2, March 1992, pp. 174-182, PMID 1557023 .

[2] S. Chen, L. Su, J. Chen, J. Wu: Cutinase: characteristics, preparation, and application. In: Biotechnology Advances . Volume 31, number 8, December 2013, pp. 1754-1767, doi : 10.1016 / j.biotechadv.2013.09.005 . PMID 24055682 .

[3] PE Kolattukudy: polyester in higher plants. In: Advances in Biochemical Engineering / Biotechnology . Volume 71, 2001, pp. 1-49, PMID 11217409 .

[4] TF Pio, GA Macedo: Cutinases: properties and industrial applications. In: Advances in applied microbiology. Volume 66, 2009, pp. 77-95, doi : 10.1016 / S0065-2164 (08) 00804-6 . PMID 19203649 .