Dihydrolipoyl transacetylase

Dihydrolipoyl transacetylase
	PDB 1Y8O
Properties of human protein
Mass / length primary structure	561 amino acids
Cofactor	2 lipoate
Identifier
Gene name	DLAT
External IDs	OMIM : 608770 ; UniProt : P10515 ; MGI : 2385311 ;
Enzyme classification
EC, category	2.3.1.12 , Transferase
Response type	Transfer of an acetyl residue
Substrate	Acetyl-CoA + Enzyme- N (6) - (Dihydrolipoyl) lysine
Products	CoA + enzyme- N (6) - ( S -acetyldihydrolipoyl) lysine
Occurrence
Parent taxon	Creature

The dihydrolipoyl transacetylase ( E2 , correctly dihydrolipoyllysine residue acetyltransferase , also dihydrolipoamide-S-acetyltransferase , PDHE2 ) is the enzyme that functions as subunit E2 of the pyruvate dehydrogenase complex (PDH) for the maintenance of the glycolysis in all living things and the citric acid cycle . Within the PDH E2 has the task of transferring of the acetyl from acetyl-CoA to the bonded PDH lipoic acid - cofactor and back. In humans, PDHE2 is the autoallergen in primarily biliary cirrhosis , an autoimmune disease . Mutations in the DLAT - gene are the cause of certain forms of pyruvate dehydrogenase deficiency .

In primary biliary cirrhosis, E2 is presumably first modified by methyl octinolate occurring in the vicinity , whereupon it becomes such a strong antigen that antibodies against normal E2 are also formed during apoptosis.

Individual evidence

↑ UniProt P10515
↑ Amano K, Leung PS, Rieger R, et al. : Chemical xenobiotics and mitochondrial autoantigens in primary biliary cirrhosis: identification of antibodies against a common environmental, cosmetic, and food additive, 2-octynoic acid . In: J. Immunol. . 174, No. 9, May 2005, pp. 5874-83. PMID 15845458 .
↑ English JS, Rycroft RJ: Allergic contact dermatitis from methyl heptine and methyl octine carbonates . In: Contact Dermatitis . 18, No. 3, March 1988, pp. 174-5. PMID 2966714 .

Web links

Wikibooks: Pyruvate Dehydrogenase Complex - Learning and Teaching Materials

Wikibooks: Biochemistry and Pathobiochemistry: Citric Acid Cycle - Learning and Teaching Materials

Citric acid cycle with pyruvate dehydrogenase position and color representations of molecules. Archived from the original on February 21, 2014 ; accessed on January 17, 2016 .
OrphaNet: Pyruvate dehydrogenase deficiency

[1] UniProt P10515

[2] Amano K, Leung PS, Rieger R, et al. : Chemical xenobiotics and mitochondrial autoantigens in primary biliary cirrhosis: identification of antibodies against a common environmental, cosmetic, and food additive, 2-octynoic acid . In: J. Immunol. . 174, No. 9, May 2005, pp. 5874-83. PMID 15845458 .

[3] English JS, Rycroft RJ: Allergic contact dermatitis from methyl heptine and methyl octine carbonates . In: Contact Dermatitis . 18, No. 3, March 1988, pp. 174-5. PMID 2966714 .