Demented
| Demented | ||
|---|---|---|
| Mass / length primary structure | 30 aa, 3513 Dalton | |
| Cofactor | Ca 2+ | |
| Identifier | ||
| External IDs | ||
| Enzyme classification | ||
| EC, category | 3.4.-.- , metalloprotease | |
| MEROPS | M9G.052 | |
| Response type | Proteolysis | |
| Substrate | Fibrinogen subunits : FGA, FGB and FGG | |
| Occurrence | ||
| Parent taxon | Hirudinea | |
Hementerin is a metalloprotease found in the saliva of a hematophagous leech called Haementeria depressa . The enzyme is responsible for anticoagulation after the bite , thus extending the duration of blood sucking on a host . It was discovered by Gastão Rosenfeld in 1955 at the Instituto Butantan in São Paulo .
function
As a protease , the enzyme is able to break down plasma proteins that play an important role in blood clotting . Fibrinogen , for example, is a plasma protein . After the enzyme has broken down the fibrin , the subunits of the fibrinogen are split: FGA ( fibrinogen alpha chain ), FGB ( fibrinogen beta chain ) and FGG ( fibrinogen gamma chain ). The lack of these fibrinogens in the blood plasma inhibits blood clotting (anticoagulation). In addition, the enzyme may be the platelet aggregation inhibiting, which by activation of nitric oxide - biosynthesis ( NO synthases ) through the collagen takes place. The fibrinolytic activity is inhibited by EDTA .
Amino acid sequence
The amino acid sequence of the hementerin looks like this according to the one -letter code :
10 20 30 XTLSEPEPTC SIEYFRYQAI EDCEYSISVK
Individual evidence
- ↑ AM Chudzinski-Tavassi, EM Kelen, AP de Paula Rosa, S. Loyau, CA Sampaio, C. Bon, E. Anglés-Cano: Fibrino (geno) lytic properties of purified hementerin, a metalloproteinase from the leech Haementeria depressa . In: Thrombosis and Haemostasis . tape 80 , no. 1 , July 1998, ISSN 0340-6245 , p. 155-160 , PMID 9684802 (English, th.schattauer.de ). th.schattauer.de ( Memento of the original from September 24, 2016 in the Internet Archive ) Info: The archive link was inserted automatically and has not yet been checked. Please check the original and archive link according to the instructions and then remove this notice.
- ↑ Ana M. Chudzinski-Tavassi, Emilse Bermej, Ruth E. Rosenstein, Fernanda Faria, María I. Keller Sarmiento, Fabiana Alberto, Misako U. Sampaio, María A. Lazzari: Nitridergic platelet pathway activation by hementerin, a metalloprotease from the leech Haementeria depressa . In: Biological Chemistry . tape 384 , no. 9 , September 2003, ISSN 1431-6730 , p. 1333-1339 , doi : 10.1515 / BC.2003.150 , PMID 14515997 (English).
- ↑ UniProt P86682
