Hoechst 33342
Structural formula | |||||||||||||||||||
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General | |||||||||||||||||||
Surname | Hoechst 33342 | ||||||||||||||||||
other names |
2 '- (4-Ethoxyphenyl) -5- (4-methyl-1-piperazinyl) -2,5'-bi-1 H -benzimidazole trihydrochloride |
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Molecular formula | C 27 H 31 Cl 3 N 6 O | ||||||||||||||||||
Brief description |
dark yellow powder |
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properties | |||||||||||||||||||
Molar mass | 561.93 (anhydrous basis) g mol −1 | ||||||||||||||||||
Physical state |
firmly |
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solubility |
soluble in water (20 mg / ml) |
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safety instructions | |||||||||||||||||||
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As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions . |
The fluorescent dye Hoechst 33342 (bisbenzimide) is used in fluorescence microscopy to stain DNA . Hoechst 33342 is excited by UV light at a wavelength of approx. 340 nm (unbound; bound 355 nm) and emits light in the spectrum from blue to cyan (approx. 510 nm). When bound to DNA, the immission maximum is 355 nm; the emission maximum at around 465 nm. By interacting with DNA, Hoechst 33342 is potentially carcinogenic and mutagenic .
Because of its low short-term toxicity, Hoechst 33342 (and similar dyes such as Hoechst 33258 or H 34580) can be used for cell count control in live cell experiments.
Web links
Individual evidence
- ↑ a b c d e data sheet bisBenzimide H 33342 trihydrochloride, ≥98% (HPLC and TLC) from Sigma-Aldrich , accessed on March 4, 2012 ( PDF ).
- ↑ SA Latt, Stetten, G, Juergens, LA, Willard, HF, Scher, CD: Recent developments in the detection of deoxyribonucleic acid synthesis by 33258 Hoechst fluorescence . In: The journal of histochemistry and cytochemistry: official journal of the Histochemistry Society . 23, No. 7, 1975 Jul, pp. 493-505. doi : 10.1177 / 23.7.1095650 . PMID 1095650 .
- ↑ a b MP Logo_master .
- ↑ Cell signal