Hoechst 33342

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Structural formula
Structural formula from Hoechst 33342
General
Surname Hoechst 33342
other names

2 '- (4-Ethoxyphenyl) -5- (4-methyl-1-piperazinyl) -2,5'-bi-1 H -benzimidazole trihydrochloride

Molecular formula C 27 H 31 Cl 3 N 6 O
Brief description

dark yellow powder

External identifiers / databases
CAS number 23491-52-3
EC number 245-691-1
ECHA InfoCard 100,041,523
PubChem 1464
ChemSpider 1420
Wikidata Q274133
properties
Molar mass 561.93 (anhydrous basis) g mol −1
Physical state

firmly

solubility

soluble in water (20 mg / ml)

safety instructions
GHS labeling of hazardous substances
05 - Corrosive 07 - Warning

danger

H and P phrases H: 302-314-335
P: 261-280-305 + 351 + 338-310
As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions .

The fluorescent dye Hoechst 33342 (bisbenzimide) is used in fluorescence microscopy to stain DNA . Hoechst 33342 is excited by UV light at a wavelength of approx. 340 nm (unbound; bound 355 nm) and emits light in the spectrum from blue to cyan (approx. 510 nm). When bound to DNA, the immission maximum is 355 nm; the emission maximum at around 465 nm. By interacting with DNA, Hoechst 33342 is potentially carcinogenic and mutagenic .

Cell nuclei of the larvae of Platynereis dumerilii stained with Hoechst 33342 .

Because of its low short-term toxicity, Hoechst 33342 (and similar dyes such as Hoechst 33258 or H 34580) can be used for cell count control in live cell experiments.

Web links

Commons : Hoechst 33342  - Collection of images, videos and audio files

Individual evidence

  1. a b c d e data sheet bisBenzimide H 33342 trihydrochloride, ≥98% (HPLC and TLC) from Sigma-Aldrich , accessed on March 4, 2012 ( PDF ).
  2. SA Latt, Stetten, G, Juergens, LA, Willard, HF, Scher, CD: Recent developments in the detection of deoxyribonucleic acid synthesis by 33258 Hoechst fluorescence . In: The journal of histochemistry and cytochemistry: official journal of the Histochemistry Society . 23, No. 7, 1975 Jul, pp. 493-505. doi : 10.1177 / 23.7.1095650 . PMID 1095650 .
  3. a b MP Logo_master .
  4. Cell signal