Hygromycin B

Structural formula
General
Surname	Hygromycin B
Molecular formula	C 20 H 37 N 3 O 13
External identifiers / databases
EC number	250-545-5
ECHA InfoCard	100.045.935
PubChem	35766
Wikidata	Q418735
properties
Molar mass	527.53 g · mol -1
safety instructions
H and P phrases	H: 300 + 310 + 330-318-334
	P: 260-264-280-301 + 310-302 + 350
	As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions .

Hygromycin B is an antibiotic substance that is produced by bacteria of the species Streptomyces hygroscopicus . It is an aminoglycoside that is effective against bacteria, fungi and cells of higher eukaryotes . The effect is based on an inhibition of protein synthesis .

history

Hygromycin B was developed in the 1950s and has since been used as a feed additive in the breeding of pigs and chickens as an anti-worm agent. Hygromycin B is not approved as a feed additive in the EU . The hygromycin B producing bacterial species Streptomyces hygroscopicus was isolated from a soil sample in 1953. Resistance genes were discovered in the early 1980s.

Use in research

In everyday laboratory work, the antibiotic is used in the selection of prokaryotic and eukaryotic cells with hygromycin resistance genes. The resistance is mediated by a kinase which inactivates hygromycin B through phosphorylation . Since the discovery of the hygromycin resistance gene, hygromycin B has been used as one of the standard antibiotics for selection.

Web links

Information from hygromycin.net (InvivoGen):

Individual evidence

↑ ^a ^b Data sheet Hygromycin B from Streptomyces hygroscopicus at Sigma-Aldrich , accessed on August 9, 2019 ( PDF ).
↑ Pettinger, RC. et al. (1953): Hygromycin I. Preliminary studies on the production and biological activity of a new antibiotic. In: Antibio. Chemother. 3 : 1268-1278.
↑ Gritz, L. and Davies, J. (1983): Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae . In: Gene 25 (2-3): 179-188; PMID 6319235 .
↑ Kaster, KR. et al. (1983): Analysis of a bacterial hygromycin B resistance gene by transcriptional and translational fusions and by DNA sequencing . In: Nucleic Acids Res. 11 (19): 6895-6911; PMID 6314265 ; PMC 326422 (free full text, PDF).
^ Rao, RN. et al. (1983): Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli . In: Antimicrob Agents Chemother . 24 (5): 689-695. PMID 6318654 ; PDF (free full text access)

[Sigma-1] Data sheet Hygromycin B from Streptomyces hygroscopicus at Sigma-Aldrich , accessed on August 9, 2019 ( PDF ).

[2] Pettinger, RC. et al. (1953): Hygromycin I. Preliminary studies on the production and biological activity of a new antibiotic. In: Antibio. Chemother. 3 : 1268-1278.

[3] Gritz, L. and Davies, J. (1983): Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae . In: Gene 25 (2-3): 179-188; PMID 6319235 .

[4] Kaster, KR. et al. (1983): Analysis of a bacterial hygromycin B resistance gene by transcriptional and translational fusions and by DNA sequencing . In: Nucleic Acids Res. 11 (19): 6895-6911; PMID 6314265 ; PMC 326422 (free full text, PDF).

[5] Rao, RN. et al. (1983): Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli . In: Antimicrob Agents Chemother . 24 (5): 689-695. PMID 6318654 ; PDF (free full text access)