New methylene blue

Structural formula
	Illustration as chloride salt
General
Surname	New methylene blue
other names	New ethylene blue; New methylene blue N; NMB; CI 52030; CI Basic Blue 24; 3,7-bis (ethylamino) -2,8-dimethylphenothiazine-5-ium chloride;
Molecular formula	C 18 H 22 ClN 3 S (chloride); C 18 H 22 ClN 3 S 0.5 ZnCl 2 (hemi-zinc chloride);
Brief description	dark brown solid (zinc chloride)
External identifiers / databases
EC number	229-516-6
ECHA InfoCard	100,026,833
PubChem	73518
ChemSpider	16736255
Wikidata	Q8214050
properties
Molar mass	347.9 g mol −1 (chloride); 416.0 g mol −1 (hemi-zinc chloride);
Physical state	firmly
solubility	4% in water, 1% in ethanol
safety instructions
GHS labeling of hazardous substances Caution
H and P phrases	H: 315-319-335
	P: 261-305 + 351 + 338
	As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions .

New methylene blue (NMB) is a phenothiazine - derivative . It is a moderately lightfast cationic dye used in medicine and is structurally similar to methylene blue .

properties

The absorption maxima of the dye in water are 591 and 630 nm . The cation is weakly lipophilic . It is commercially available as a double salt with zinc chloride , but has also been sold as a chloride salt. In some cases, brilliant cresyl blue is also marketed as “new methylene blue”.

use

In addition to brilliant cresyl blue, NMB is used for diagnostic staining ( vital staining ) of unripened red blood cells in histopathology and cytopathology.

It was originally used as a metachromatic dye for mucins in the 1940s , and in the 1990s, among other things. a. for microscopic staining of Heinz bodies or as a cytogenetic micronucleus test .

literature

Richard W. Horobin, John A. Kiernan: Conn's Biological Stains: A Handbook of Dyes, Stains and Fluorochromes for Use in Biology and Medicine. 10th edition. BIOS Scientific Publ., Oxford 2002, ISBN 1-85996-099-5 , pp. 300 f.

Individual evidence

↑ ^a ^b ^c Entry on new methylene blue N zinc chloride double salt in the GESTIS substance database of the IFA , accessed on January 10, 2017(JavaScript required) .
↑ Richard W. Horobin, John A. Kiernan: Conn's Biological Stains: A Handbook of Dyes, Stains and Fluorochromes for Use in Biology and Medicine . 10th edition, Oxford, BIOS Scientific, 2002, ISBN 978-1-85996-099-8 , p. 300.
^ Jürgen Hallbach: Clinical chemistry and hematology: Biomedical analysis for MTLA and studies. 3. Edition. Thieme Verlag, 2011, ISBN 978-3-13-106343-4 , p. 410.

[GESTIS-1] Entry on new methylene blue N zinc chloride double salt in the GESTIS substance database of the IFA , accessed on January 10, 2017(JavaScript required) .

[2] Richard W. Horobin, John A. Kiernan: Conn's Biological Stains: A Handbook of Dyes, Stains and Fluorochromes for Use in Biology and Medicine . 10th edition, Oxford, BIOS Scientific, 2002, ISBN 978-1-85996-099-8 , p. 300.

[3] Jürgen Hallbach: Clinical chemistry and hematology: Biomedical analysis for MTLA and studies. 3. Edition. Thieme Verlag, 2011, ISBN 978-3-13-106343-4 , p. 410.