Nisin
| Nisin ( Lactococcus lactis subsp. Lactis ) | ||
|---|---|---|
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| Rod model according to PDB 1WCO | ||
| Mass / length primary structure | 34 amino acids | |
| Precursor | (57 aas) | |
| Identifier | ||
| Gene name (s) | spaN; nisA | |
| External IDs |
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| Transporter classification | ||
| TCDB | 1.C.20.1.1 | |
| designation | Nisin family | |
| Occurrence | ||
| Homology family | Nisin / subtilin | |
| Parent taxon | Bacillus / Lactobacillus / Streptococcus group | |
Nisin is an antibiotic peptide from the group of lantibiotics ( antibiotics containing lanthionine ). It has a broad spectrum of activity against gram-positive bacteria . Nisin is produced by the lactic acid bacterium Lactococcus lactis . It is found in raw milk and is considered harmless to healthy people because it is believed to be broken down by digestive enzymes in a short time.
Because of its antibiotic effect (only against gram-positive bacteria ), nisin is suitable for preservation . It is approved in most European countries as a preservative for various foods ( pudding , cheese , processed cheese ). As a food additive , it has the number E 234.
Nisin is also used as an antibiotic in human and veterinary medicine .
Nisin belongs to the nisin family of pore-forming toxins . It uses Lipid II in the bacterial cell membrane to open a pore with a diameter of 2 to 2.5 nanometers for several seconds. It is a class I lantibiotic .
Analytics
Coupling of HPLC with mass spectrometry or ELISA tests can be used for reliable qualitative and quantitative determination of nisin in the various test materials.
Individual evidence
- ↑ a b K. A. Stevens et al: Nisin treatment for inactivation of Salmonella species and other gram-negative bacteria. In: Applied and Environmental Microbiology. Volume 57, No. 12, 1991, pp. 3613-3615.
- ↑ a b L. T. Cao, JQ Wu, F. Xie, SH Hu, Y. Mo: Efficacy of nisin in treatment of clinical mastitis in lactating dairy cows. In: Journal of dairy science. Volume 90, No. 8, 2007, pp. 3980-3985.
- ↑ a b A. H. Soomro, T. Masud, K. Anwaar: Role of lactic acid bacteria (LAB) in food preservation and human health - a review. ( Memento of the original from October 19, 2013 in the Internet Archive ) Info: The archive link was inserted automatically and has not yet been checked. Please check the original and archive link according to the instructions and then remove this notice. In: Pakistan Journal of Nutrition. Volume 1, No. 1, 2002, pp. 20-24, PDF.
- ↑ ZVerkV - single standard - Annex 2 (to § 3 Paragraph 1) Sales names and purity requirements for additives approved for technological purposes ( Memento of February 11, 2013 in the web archive archive.today )
- ↑ Clara Aranha, Sadhana Gupta, KVR Reddy: Contraceptive efficacy of antimicrobial peptide nisin: in vitro and in vivo studies. In: Contraception. Volume 69. No. 4, 2004, pp. 333-338.
- ↑ TCDB entry
- ↑ KY Ko, SR Park, CA Lee, M. Kim: Analysis method for determination of nisin A and nisin Z in cow milk by using liquid chromatography-tandem mass spectrometry. In: J Dairy Sci. 98 (3), Mar 2015, pp. 1435-1442. PMID 25529415
- ^ N. Schneider, K. Werkmeister, M. Pischetsrieder: Analysis of nisin A, nisin Z and their degradation products by LCMS / MS. In: Food Chem. 127 (2), Jul 15, 2011, pp. 847-854. PMID 23140745
- ↑ S. Aly, J. Floury, MH Famelart, MN Madec, D. Dupont, Y. Le Gouar, p Lortal, p Jeanson: nisin quantification by ELISA Allows the modeling of its apparent diffusion coefficient in model cheeses. In: J Agric Food Chem. 59 (17), Sep 14, 2011, pp. 9484-9490. PMID 21770386
literature
- Cortina Kaletta, Karl-Dieter Entian: Nisin, a Peptide Antibiotic: Cloning and Sequencing of the nisA Gene and Posttranslational Processing of its Peptide Product. (PDF; 1.0 MB) In: J. Bact. 1989, pp. 1597-1601.