Picro Sirius red staining

from Wikipedia, the free encyclopedia
Picro Sirius red staining of fibrosis in MDP syndrome

The pikro-Sirius Red staining is a histological technique for staining of collagen in tissues .

principle

With the picro-Sirius red staining, collagen red and muscle fibers , cytoplasm and the background yellow appear under the light microscope . With polarization microscopy , collagen fibers of type I (thick fibers) show a yellow-orange and those of type III (thin fibers) a green birefringence on. The staining solution consists of Sirius red (1 g / L of the derivatives F3B or F3A) in a saturated aqueous solution of picric acid . Sirius red is a comparatively large dye molecule that causes an increase in birefringence when it attaches along collagen fibers (optical anisotropy ). As a large dye molecule , it diffuses only slowly into denser tissue structures, which means that tissue that does not consist of collagen is only slightly stained after a short staining time of 1 to 2 hours. The exact mechanism of action of picric acid is unknown; it increases the optical anisotropy of the Sirius red color. The staining solution can be kept for over a month. Dried picric acid is explosive .

Alternatively, the Van Gieson stain , the Masson trichrome stain and the Mallory trichrome stain can be used in part.

history

Sirius red was first used in 1964 by F. Sweat and H. Puchtler for dyeing fabrics. The picro Sirius red stain was first used in 1968 by V. Constantine and R. Mowry. The staining with Sirius red was more lightfast than the previously used staining with picro- fuchsin .

literature

Individual evidence

  1. ^ Lillian Rich, Peter Whittaker: Collagen and Picrosirius Red Staining: A polarized light assessment of fibrillar hue and spatial distribution . In: Braz. J. morphol. Sci. (2005), Volume 22, Issue 2, pp. 97-104.
  2. ↑ R. Lattouf, R. Younes, D. Lutomski, N. Naaman, G. Godeau, K. Senni, p Changotade: picrosirius Red Staining: A Useful Tool to Appraise Collagen Networks in Normal and Pathological tissue. ( Memento of the original from March 9, 2016 in the Internet Archive ) Info: The archive link was inserted automatically and has not yet been checked. Please check the original and archive link according to the instructions and then remove this notice. In: Journal of Histochemistry & Cytochemistry. 62, 2014, p. 751, doi : 10.1369 / 0022155414545787 . @1@ 2Template: Webachiv / IABot / jhc.sagepub.com
  3. ↑ a b c Kyriacos A. Athanasiou: Articular Cartilage. CRC Press, 2013, ISBN 978-1-439-85324-5 , p. 330.
  4. ↑ Data sheet Picro-Sirius Staining: pdf ( Memento of the original from March 15, 2016 in the Internet Archive ) Info: The archive link was inserted automatically and has not yet been checked. Please check the original and archive link according to the instructions and then remove this notice. ; last accessed on March 15, 2016. @1@ 2Template: Webachiv / IABot / www.dianova.com
  5. ^ A b John Varga: Fibrosis Research. Springer Science & Business Media, 2005, ISBN 978-1-592-59940-0 , pp. 180, 275.
  6. ^ Hans Gregersen: Biomechanics of the Gastrointestinal Tract. Springer Science & Business Media, 2013, ISBN 978-1-447-13742-9 .
  7. ↑ Massimo Sabatini, Frédéric De Ceuninck, Philippe Pastoureau: Cartilage and Osteoarthritis , Springer Science & Business Media, 2004, p. 54, ISBN 9781592598212 , here online
  8. ^ A b Laszlo Modis: Organization of the Extracellular Matrix. CRC Press, 1990, ISBN 978-0-849-35786-2 , p. 58.
  9. ↑ F. SWEAT, H. PUCHTLER, SI ROSENTHAL: SIRIUS RED F3BA AS A STAIN FOR CONNECTIVE TISSUE. In: Archives of pathology. Volume 78, July 1964, pp. 69-72, PMID 14150734 .
  10. ↑ a b V.S. Constantine, RW Mowry: Selective Staining of Human Dermal Collagen. In: Journal of Investigative Dermatology. 50, 1968, p. 419, doi : 10.1038 / jid.1968.68 .